F. Zhou

Unbiased functional genetic screens reveal essential RNA modifications in human cancer and drug resistance

The paper identifies essential RNA modifications in cancer using CRISPR screens and finds that mitochondrial metabolic plasticity influences drug resistance through specific RNA modifications.

Filament structures unveil the dynamic organization of human acetyl-CoA carboxylase

The paper determines cryo-EM structures of human ACC1 in inactive and activated states, revealing structural dynamics and regulatory mechanisms.

Multi-parametric thrombus profiling microfluidics detects intensified biomechanical thrombogenesis associated with hypertension and aging

This paper introduces a microfluidics-based thrombus profiling assay that detects intensified biomechanical thrombogenesis in hypertension and aging, revealing a GPIb-integrin mechanosensing axis hyperactivity mechanism.

Anchorage-independent cell proliferation promoted by fascin's F-actin bundling

This study identifies fascin-1's role in promoting anchorage-independent proliferation through a novel signaling complex called PASS, which drives Rac1/PAK/JNK signaling in cancer cells.

Structural insights into human propionyl-CoA carboxylase (PCC) and 3-methylcrotonyl-CoA carboxylase (MCC)

The paper reports high-resolution cryo-EM structures of human PCC and MCC, revealing substrate binding modes and biotin coordination mechanisms.

Targeting GFPT2 to reinvigorate immunotherapy in EGFR-mutated NSCLC

This study identifies GFPT2 as a metabolic checkpoint in EGFR-mutated NSCLC that promotes immune evasion, and shows that targeting GFPT2 enhances PD-1 blockade therapy.

LRRK2 G2019S mutation suppresses differentiation of Th9 and Treg cells via JAK/STAT3

This study shows that the LRRK2 G2019S mutation impairs Th9 and Treg cell differentiation while enhancing Th2 differentiation through JAK/STAT3 signaling in T cells.

Magnesium Transporter MgtA revealed as a Dimeric P-type ATPase

The paper reports the first structure of the Mg2+ transporter MgtA using cryo-EM, revealing its dimeric form and mechanism of Mg2+ transport.

Mechanical confinement induces ferroptosis through mitochondrial dysfunction

This study shows that mechanical confinement triggers ferroptosis via mitochondrial dysfunction by inducing Drp1-dependent fragmentation and cPLA2 translocation.

TLR9 activation in large wound induces tissue repair and hair follicle regeneration via gamma delta T cells

This study shows that TLR9 activation in wounds promotes tissue repair and hair follicle regeneration through gamma delta T cells by increasing AREG production and modulating keratinocyte fate.

A Prodrug Strategy to Reposition Atovaquone as a Long-Acting Injectable for Malaria Chemoprotection

This paper develops three prodrug formulations of atovaquone for long-acting injectable malaria chemoprotection, showing two maintain therapeutic plasma levels for over 70 and 30 days in monkeys.

Multiple layers of innate immune response antagonism of SARS-CoV-2

This study generates twelve SARS-CoV-2 viruses with mutations in individual proteins and shows that many have interferon-antagonizing activity and immunosuppressive effects in human cells and mice, with NSP1 and NSP15 having the greatest impact.

Regulatory T cells and IFN-γ-producing Th1 cells play a critical role in the pathogenesis of Sjögren's Syndrome

This study identifies Treg cells and IFN-γ-producing Th1 cells as key drivers of Sjögren's Syndrome pathogenesis using a mouse model and single-cell transcriptomics.

The lipid-mediated mechanism of mechanosensitive channel MscS inactivation

This study identifies a lipid-mediated mechanism by which the mechanosensitive channel MscS inactivates, showing that intercalated lipids physically decouple tension-sensing helices from the gate.

Loss of STIM1 and STIM2 in salivary glands disrupts ANO1 function but does not induce Sjogren's disease

This study shows that loss of STIM1 and STIM2 in salivary glands reduces ANO1 function and saliva production but does not cause Sjogren's disease, suggesting a link between SOCE and TLR8 signaling.

Obesity status and its relative factors of captive Asian elephants (Elephas maximus) in China based on body condition assessment

The paper assesses obesity in captive Asian elephants in China by analyzing body condition and related factors, finding over 70% are overweight or obese, with insufficient outdoor time linked to obesity.

USP7 Inhibition Promotes Early Osseointegration in Senile Osteoporotic Mice

This study shows that USP7 inhibition accelerates osseointegration in senile osteoporotic mice by enhancing macrophage efferocytosis and inducing apoptosis in aged BMSCs.

Urine proteomic analysis of patients with schizophrenia

This study identifies 35 differential urinary proteins between unmedicated schizophrenia patients and healthy controls, with 15 linked to schizophrenia mechanisms, showing potential as diagnostic markers.

Regulated Cleavage and Relocation of FERONIA Control Immunity in Arabidopsis Roots

Bacteria induce FER cleavage via At2-MMP to trigger localized immunity in Arabidopsis root transition/elongation zones.

Transcriptome-wide analysis of the function of Ded1 in translation preinitiation complex assembly in a reconstituted in vitro system

This study uses Rec-Seq to show Ded1 promotes 48S PIC formation on mRNAs with long 5UTRs and that eIF4A is required for PIC assembly on all mRNAs.

Prolonging the Delivery of Influenza Virus Vaccine Improves the Quantity and Quality of the Induced Immune Responses in Mice

This study shows that prolonged influenza vaccine delivery in mice enhances both the quantity and quality of immune responses compared to prime-boost immunization.

Cryo-EM structures of human magnesium channel MRS2 reveal gating and regulatory mechanisms

This study determines the cryo-EM structures of human MRS2 with and without Mg2+ and identifies key residues and binding sites involved in Mg2+ regulation.

CCR9 overexpression promotes T-ALL progression by enhancing cholesterol biosynthesis

This study compares the tumorigenic potential of MOLT4 and JURKAT T-ALL cell lines, finding that CCR9 overexpression enhances cholesterol biosynthesis and promotes T-ALL progression.

A critical role of VEGFR2 in lymphatic tumor metastasis

This study shows that VEGFR2 in lymphatic endothelial cells promotes tumor metastasis by regulating lymphatic valve formation and tumor-associated lymphangiogenesis.

Structure of human NaV1.6 channel reveals Na+ selectivity and pore blockade by 4,9-anhydro-tetrodotoxin

The paper presents cryo-EM structures of human NaV1.6 channels with and without 4,9-anhydro-tetrodotoxin, revealing Na+ selectivity and inhibition mechanisms.

Structure-Kinetic Relationship for Drug Design Revealed by PLS Model with Retrosynthesis-Based Pre-trained Molecular Representation and Molecular Dynamics Simulation

This paper develops a PLS model combining retrosynthesis-based molecular representations and MD simulations to predict koff values for HSP90 inhibitors, showing high correlation with experimental data.

Intranasal self-amplifying RNA SARS-CoV-2 vaccine produces protective respiratory and systemic immunity and prevents viral transmission

This study compares intranasal and intramuscular delivery of a self-amplifying RNA SARS-CoV-2 vaccine, showing that intranasal delivery induces mucosal immunity and reduces viral transmission.

Resolution doubling in light-sheet microscopy via oblique plane structured illumination

This paper demonstrates isotropic resolution doubling in light-sheet microscopy using oblique plane structured illumination, achieving sub-150nm lateral resolution with reduced phototoxicity and faster volumetric imaging.

Tumor microenvironments impair T cell receptor affinity and function

This study shows that CD8+ T cells in the tumor microenvironment have reduced TCR-pMHC affinity and function, impairing their anti-tumor activity.

Computational pipeline provides mechanistic understanding of Omicron variant of concern neutralizing engineered ACE2 receptor traps

The paper develops a computational pipeline combining Rosetta and cryo-EM to analyze ACE2 receptor traps' binding to SARS-CoV-2 variants, showing high affinity and neutralization capabilities against Omicron and Delta.