E. Stefan

GPR161 structure uncovers the redundant role of sterol-regulated ciliary cAMP signaling in the Hedgehog pathway

The paper determines the cryo-EM structure of active GPR161 bound to Gs, revealing sterol binding and its role in ciliary cAMP signaling, while showing that sterol binding is not essential for Hedgehog pathway suppression.

Kinases in motion: impact of protein and small molecule interactions on kinase conformations

This study uses KinCon-reporter technology to analyze conformational changes in disease-associated kinases upon drug, regulatory protein, and pathway activation interactions, revealing insights into therapeutic strategies.

A comprehensive study of SARS-CoV-2 main protease (Mpro) inhibitor-resistant mutants selected in a VSV-based system

This study identifies and characterizes SARS-CoV-2 Mpro mutations conferring resistance to nirmatrelvir using a VSV-based system and biochemical assays.

DISRUPTOR: Computational identification of oncogenic mutants disrupting protein interactions

DISRUPTOR identifies oncogenic mutations disrupting protein interactions using an Osprey-based protocol, validated on clinically relevant mutations and confirmed experimentally.

G3BP1 tethers the TSC complex to lysosomes and suppresses mTORC1 in the absence of stress granules

This paper shows G3BP1 anchors the TSC complex to lysosomes to suppress mTORC1 signaling without stress granules, and inhibits mTORC1-driven cancer and neuronal issues.

Non-oncology drugs are a source of previously unappreciated anti-cancer activity

The paper identifies non-oncology drugs with anti-cancer activity across 578 cancer cell lines using PRISM, finding that many drugs selectively inhibit cancer cells based on molecular features.

Zebrafish Gpr161 contributes to basal hedgehog repression in a tissue-specific manner

This study shows that Gpr161 contributes to tissue-specific basal repression of Hedgehog signaling in zebrafish by modulating PKA activity in the neural tube and myotome.