H. B. Bode

Identification, structure and function of the methyltransferase involved in the biosynthesis of the dithiolopyrrolone antibiotic xenorhabdin

This study identifies and characterizes XrdM, an amide methyltransferase involved in dithiolopyrrolone antibiotic biosynthesis, and shows it is distinct from DtpM in structure and function.

Genetic Toolbox for Photorhabdus and Xenorhabdus: pSEVA based heterologous expression systems and CRISPR/Cpf1 based genome editing for rapid natural product profiling

This study develops new genetic tools for Photorhabdus and Xenorhabdus, including SEVA-based expression vectors and CRISPR/Cpf1 genome editing systems, enabling efficient natural product profiling.

Transertion and cell geometry organize the Escherichia coli nucleoid during rapid growth

This study shows that transertion and cell geometry influence the spatial organization of the E. coli nucleoid during growth and antibiotic treatment.

Biosynthesis of the Multifunctional Isopropylstilbene in Photorhabdus laumondii Involves Cross-Talk between Specialized and Primary Metabolism

This study identifies the role of StlB in IPS biosynthesis and demonstrates cross-talk between primary and specialized metabolism, enabling first-time IPS production in a heterologous host.

Global analysis of biosynthetic gene clusters reveals conserved and unique natural products in entomopathogenic nematode-symbiotic bacteria

The paper analyzes biosynthetic gene clusters in entomopathogenic nematode-symbiotic bacteria to identify conserved and unique natural products and their functional roles.

Type S Non Ribosomal Peptide Synthetases for the rapid generation of tailor-made peptide libraries

This paper describes the use of type S non-ribosomal peptide synthetases to generate diverse peptide libraries efficiently, producing 49 peptides and derivatives at high titers.

Influence of condensation domains on activity and specificity of adenylation domains

The paper investigates the role of the C-A interface in NRPSs, showing that the C domain's influence on activity and specificity is mediated through its interaction with the A domain, not directly.

The Chemical Structure of Widespread Microbial Aryl Polyene Lipids

This study identifies the chemical structure of aryl polyene lipids (APELs) from Xenorhabdus doucetiae using chromatographic purification and structural analysis techniques.

Small RNA directs symbiosis, virulence, and natural products biosynthesis in entomopathogenic bacteria

This study identifies ArcZ, an Hfq-dependent sRNA, as essential for small molecule production in entomopathogenic bacteria, revealing its broad regulatory role beyond secondary metabolites.

Cell-free synthesis of natural compounds from genomic DNA of biosynthetic gene clusters

This study demonstrates that cell-free systems can be used to produce and characterize multidomain biosynthetic proteins for natural compound synthesis.

Focused natural product elucidation by prioritizing high-throughput metabolomic studies with machine learning

The paper uses machine learning to prioritize metabolites in high-throughput metabolomic studies of Photorhabdus and Xenorhabdus bacteria, identifying previously unknown compounds.

Structure-based redesign of docking domain interactions modulates the product spectrum of a rhabdopeptide-synthesizing NRPS

The paper identifies structural and thermodynamic rules governing docking domain interactions in NRPSs, enabling predictable changes in peptide product spectra through residue exchange.