R. Henriques

Transertion and cell geometry organize the Escherichia coli nucleoid during rapid growth

This study shows that transertion and cell geometry influence the spatial organization of the E. coli nucleoid during growth and antibiotic treatment.

This microtubule does not exist: Super-resolution microscopy image generation by a diffusion model

This paper trains a diffusion model to generate realistic super-resolution microscopy images and shows its effectiveness in improving deconvolution performance with limited real data.

Processive movement of Staphylococcus aureus essential septal peptidoglycan synthases is independent of FtsZ treadmilling and drives cell constriction

This study shows that FtsW/PBP1 and DivIB move processively around the division site in S. aureus, driving cell constriction independently of FtsZ treadmilling.

CLEM-Reg: An automated point cloud based registration algorithm for correlative light and volume electron microscopy

CLEM-Reg automates 3D alignment of vCLEM datasets, achieving sub-micron correlation of fluorescent signals with EM structures and enabling identification of TGN46-positive transport carriers.

High-fidelity 3D live-cell nanoscopy through data-driven enhanced super-resolution radial fluctuation

This paper introduces eSRRF, an enhanced super-resolution imaging method that improves image fidelity and extends to 3D imaging using multi-focus microscopy.

TOR acts as metabolic gatekeeper for auxin-dependent lateral root initiation in Arabidopsis thaliana

The paper shows that TOR kinase regulates auxin-dependent lateral root initiation in Arabidopsis by modulating translation of key auxin targets and integrating metabolic signals.

Nanoscale colocalization of NK cell activating and inhibitory receptors controls signal integration

This study shows that nanoscale colocalization of NK cell activating and inhibitory receptors determines signal integration and inhibition.

DeepBacs: Bacterial image analysis using open-source deep learning approaches

DeepBacs uses open-source deep learning to analyze bacterial microscopy images, offering tools for segmentation, classification, and phenotyping with applications in antibiotic research.

Physical mechanisms of ESCRT-III-driven cell division in archaea

The paper develops a physical model to explain how ESCRT-III filaments drive cell division in archaea, showing that active geometry changes and disassembly are sufficient for efficient splitting.

Single-molecule super-resolution imaging of T-cell plasma membrane CD4 redistribution upon HIV-1 binding

This study uses single-molecule super-resolution imaging to quantify nanoscale CD4 redistribution on CD4+ T cells after HIV-1 binding, revealing cluster size and molecule number changes.

Live cell imaging of the hyperthermophilic archaeon Sulfolobus acidocaldarius identifies complementary roles for two ESCRTIII homologues in ensuring a robust and symmetric cell division

The paper develops a heated imaging system to study Sulfolobus cell division, revealing distinct roles for two ESCRTIII proteins in ensuring robust and symmetric division.

Super-Beacons: open-source probes with spontaneous tuneable blinking compatible with live-cell super-resolution microscopy

The paper introduces Super-Beacons, open-source DNA-based probes that enable spontaneous photoswitching for live-cell super-resolution microscopy without high-intensity illumination.

vLUME: 3D Virtual Reality for Single-molecule Localization Microscopy

vLUME is a VR software for visualizing 3D SMLM data, enabling segmentation and quantification of fluorescence puncta with high fidelity.

Closed mitosis requires local disassembly of the nuclear envelope

This study identifies a novel protein involved in local nuclear envelope breakdown during closed mitosis in fission yeast.

Proteasome-mediated protein degradation resets the cell division cycle and triggers ESCRT-III-mediated cytokinesis in an archaeon

This study identifies proteasome-mediated degradation of CdvB as a mechanism resetting the cell division cycle and triggering cytokinesis in the archaeon Sulfolobus acidocaldarius.

Fluctuation-based Super-Resolution Traction Force Microscopy

This paper introduces a method to improve traction force microscopy resolution and throughput using super-resolution microscopy based on fluorophore fluctuation analysis.

Beyond parallel evolution: when several species colonize the same environmental gradient

The paper examines genomic divergence in four flatfish species colonizing the North Sea-Baltic Sea gradient, finding heterogeneous divergence patterns and no parallel genomic architecture.

Nuclear pores as versatile reference standards for quantitative superresolution microscopy

The paper uses genome-edited cell lines with labeled nuclear pore complexes to serve as reference standards for quantitative superresolution microscopy.

Super-resolution fight club: A broad assessment of 2D & 3D single-molecule localization microscopy software

The paper evaluates 36 single-molecule localization microscopy software packages using simulated datasets to assess their performance in 2D and 3D imaging.

Fix your membrane receptor imaging: Actin cytoskeleton and CD4 membrane organization disruption by chemical fixation

The paper shows that fixation methods affecting the actin cytoskeleton alter CD4 membrane organization in super-resolution microscopy.

Nanoscale Polarization of the Vaccinia Virus Entry Fusion Complex Drives Efficient Fusion

This study uses super-resolution microscopy to show that vaccinia virus fusion proteins are polarized at virion tips, and disrupting this polarization reduces fusion efficiency.

Automating multimodal microscopy with NanoJ-Fluidics

The paper introduces NanoJ-Fluidics, an open-source system for automating multimodal microscopy workflows, demonstrating its ability to perform complex imaging experiments with high reproducibility.

TMEM16F activation by Ca2+ triggers plasmalemma expansion and directs PD-1 trafficking

This study shows TMEM16F activation by Ca2+ causes surface membrane expansion and PD-1 trafficking in T cells.

A hidden Markov model approach to characterizing the photo-switching behavior of fluorophores

The paper uses a hidden Markov model to characterize fluorophore photo-switching behavior by estimating transition rates from image sequences.

NanoJ-SQUIRREL: quantitative mapping and minimisation of super-resolution optical imaging artefacts

NanoJ-SQUIRREL quantitatively maps and minimizes super-resolution imaging artefacts by comparing diffraction-limited and super-resolution images across various techniques.