M. Grininger

The Kinetics of Carbon-Carbon-Bond Formation in Metazoan Fatty Acid Synthase and its Impact on Product Fidelity

The paper investigates the kinetics of carbon-carbon bond formation in fatty acid synthase, revealing how the enzyme's activity and fidelity are influenced by substrate length and dimeric cooperativity.

Docking Domain Engineering in a Modular Polyketide Synthase and its Impact on Structure and Function

This study engineers docking domains in modular PKSs to couple polypeptides, showing that high-affinity interactions can improve synthesis but may reduce rates, with efficiency recovery through hinge regions.

Directed biosynthesis of fluorinated polyketides

This paper describes a method to directly fluorinate polyketides using a modified PKS enzyme, demonstrating its application in creating a fluorinated antibiotic derivative.

Comparative Study on Acyl Transferases in Fatty Acid and Polyketide Synthases

This study compares acyl transferase domains in fatty acid and polyketide synthases, finding modular PKS ATs are slower and more specific than iterative ones, with detailed analysis of DEBS AT interactions.

Cell-free synthesis of natural compounds from genomic DNA of biosynthetic gene clusters

This study demonstrates that cell-free systems can be used to produce and characterize multidomain biosynthetic proteins for natural compound synthesis.

Dodecin as carrier protein for immunizations and bioengineering applications

This paper introduces dodecin as a scaffold protein for immunization and bioengineering, demonstrating its ability to carry and deliver cargo for antibody production.

The Ketosynthase Domain Constrains the Design of Polyketide Synthases

This study identifies bottlenecks in chimeric modular PKSs and proposes guidelines for improving their efficiency through targeted mutations in the ketosynthase domain.

The resolution revolution in cryoEM requires new sample preparation procedures: A rapid pipeline to high resolution maps of yeast FAS

The paper introduces a rapid pipeline for producing high-resolution cryoEM maps of yeast FAS, achieving a 3.1 Å map from 15,000 particles in one day.

Type I fatty acid synthase (FAS) trapped in the octanoyl-bound state

The paper presents the crystal structure of murine FAS in an octanoyl-bound state, revealing structural dynamics and substrate specificity that inform inhibitor design.

Engineering of chimeric polyketide synthases using SYNZIP docking domains

This study compares chimeric PKSs with different interfaces to identify the least invasive method for PKS engineering, showing that SYNZIP domains can effectively replace natural docking domains.

The deadly touch: protein denaturation at the water-air interface and how to prevent it

The paper investigates protein denaturation at the air-water interface using electron cryo-microscopy and shows that hydrophilized graphene prevents this denaturation.

Probing the Modularity of Megasynthases by Rational Engineering of a Fatty Acid Synthase (FAS) Type I

This study re-engineers animal fatty acid synthase (FAS) to probe modularity in FAS/PKS family, creating new PKS-like modules with enhanced catalytic efficiency.

Analysis and Engineering of Substrate Shuttling by the Acyl Carrier Protein (ACP) in Fatty Acid Synthases (FASs)

This study assesses how domain-domain interactions affect fatty acid synthesis by mutating interfaces in FASs and measuring product spectra and enzyme activity.

Site-Specific Labelling of Multidomain Proteins by Amber Codon Suppression

The paper investigates the use of amber codon suppression to label multidomain proteins, demonstrating its applicability through a microplate-based assay and conformational analysis of murine fatty acid synthase.