Hao Wu

Fab Dimerization Crosslinks and Activates the γδ T-Cell Receptor

This study presents the structures of the Vγ9Vδ2 TCR in inactive and agonist-crosslinked states, revealing a dimerization-driven activation mechanism.

Chimeric archaeal BHB-human pre-tRNA scaffold HARBOR system enables versatile production of functional circular RNA modalities

The paper introduces the HARBOR system for producing functional circular RNAs with high efficiency and versatility in both cells and vivo.

Integrating complementary biological information for multi-objective enzyme engineering

This study develops a machine learning framework to engineer enzymes by integrating sparse data with evolutionary and structural information, achieving improvements in catalytic performance, yield, and stability.

Brain deviation scores derived from normative modelling identify dimensions of psychopathology in children and adolescents

The paper uses normative modeling to derive brain deviation scores and identifies an emotion-dysregulation dimension in children and adolescents linked to psychiatric symptoms.

ATP-dependent reorientation on a nucleosome primes INO80 for DNA translocation

This study reveals that INO80 reorients on a nucleosome upon ATP hydrolysis, enabling DNA translocation through a unique mechanism distinct from other chromatin remodelers.

CryoROLE: describing large inter-domain rotation in single particle cryo-EM

CryoROLE extracts continuous conformational dynamics from cryo-EM data by analyzing relative domain orientations, revealing hidden protein motions relevant to function.

Granulosa cell glycogen fuels the avascular corpus luteum

This study identifies GC energy storage as a mechanism enabling avascular corpus luteum function by glycogen catabolism, with implications for reproductive health.

Chromatin topology control by a muscle-specific ribosomal protein

This study identifies Rpl3l as a muscle-specific ribosomal protein that regulates chromatin insulation in atrial cardiomyocytes, linking genome structure to cardiac rhythm stability.

Multimodal profiling reveals the centromedian nucleus of thalamus as a dynamic hub orchestrating staged consciousness recovery

This paper develops a multiscale framework to decode consciousness recovery from anesthesia into three stages and identifies the centromedian nucleus of thalamus as a dynamic hub orchestrating this process.

HYPER-Net: Physics-Conditioned Self-Supervised Reconstruction for Fourier Light-Field Microscopy

HYPER-Net enables physics-conditioned self-supervised reconstruction for Fourier light-field microscopy, improving volumetric imaging across diverse biological contexts.

Steady-state epithelial apical flatness is characterized by MLCK morphodynamics and asynchronous Ca2+ oscillations, but not underlying ECM geometry

This study shows that apical flatness in epithelial tissues is maintained by MLCK-driven myosin II dynamics and asynchronous Ca2+ oscillations, independent of basement membrane geometry.

In vivo-directed evolution identifies AAV-WM04 as a next-generation vector for potent and durable hearing restoration in DFNB9

This study identifies AAV-WM04 as a highly efficient and safe gene delivery vector for targeting inner hair cells in the cochlea, showing effectiveness in mice and non-human primates for treating hereditary hearing loss.

Primate lineage specification requires suppression of Alu hyperediting

The paper identifies ILF2/3 as a regulator of primate cell fate by suppressing Alu element editing, showing its role in preventing aberrant splicing and maintaining epigenetic stability.

Nonlinear dynamic genetic regulation identifies peripheral drivers of neurodegenerative disease progression

This study identifies peripheral genetic drivers of neurodegenerative disease progression using a novel two-stage framework linking genetic variants to longitudinal molecular traits and disease phenotypes.

Human Messenger RNA Harbors Widespread Noncoding Splice Isoforms

The paper identifies 15,836 noncoding splice isoforms in human mRNA from 7,298 protein-coding genes, showing tissue-specific expression and association with cancer.

A novel dephosphorylation peptide inhibits 17β-HSD1 enzyme activity in ovarian granulosa cells and breast cancer cells

This study identifies phosphorylation sites in 17β-HSD1 and develops cell-penetrating peptides that inhibit its activity, reducing breast cancer cell proliferation and tumor growth.

Stress-induced epigenetic regulation of transcription in neocortical excitatory neurons drives depression-like behavior

The paper identifies epigenetic changes in neocortical excitatory neurons under chronic stress that contribute to depression-like behaviors in mice.

Predictive modeling of single-cell DNA methylome data enhances integration with transcriptome data

The paper introduces MAPLE, a framework that predicts gene activity from single-cell DNA methylation data, improving integration with transcriptome data for clustering and cell type identification.

Single cell transcriptomic analysis of the adult mouse pituitary reveals a novel multi-hormone cell cluster and physiologic demand-induced lineage plasticity

This study uses scRNA-seq to reveal a novel multi-hormone cell cluster and lineage plasticity in the adult mouse pituitary under physiological stress.

sNucDrop-Seq: Dissecting cell-type composition and neuronal activity state in mammalian brains by massively parallel single-nucleus RNA-Seq

sNucDrop-Seq isolates nuclei from adult mouse brains to analyze cell-type composition and neuronal activity states at single-nucleus resolution.

Molecular signatures associated with ZIKV exposure in human cortical neural progenitors

This study identifies virus- and strain-specific molecular signatures of ZIKV infection in human cortical neural progenitors, showing distinct impacts on DNA replication and apoptosis.