J. Wu

SplitAligner: A Gene-Species Tree Reconciliation Framework Using Split-Based Branch Mapping

SplitAligner evaluates branch-specific concordance across genes in a species tree, identifying missingness and discordance in phylogenomic data.

A Host Enzyme Reduces Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) by Inactivating Intestinal Lipopolysaccharide

This study shows that AOAH inactivates intestinal LPS, reducing MASLD by preventing hepatic lipid accumulation and altering lipid metabolism gene expression.

A high resolution model of the grapevine leaf morphospace predicts synthetic leaves

The paper develops a high-resolution morphospace model of grapevine leaves to predict synthetic leaves and improve classification accuracy.

Refinement of the Brassica napus NLRome using RenSeq

This study refines the NLRome of Brassica napus using RenSeq, increasing annotated NLR genes from 345 to 715 and identifying genomic regions with misannotations.

Shrimp Hemocyanin elicits a potent humoral response in mammals and is favorable to hapten conjugation

This study shows shrimp hemocyanin (SHC) can replace keyhole limpet hemocyanin (KLH) as a carrier protein for hapten conjugation, offering better solubility and comparable immunogenicity in mammals.

SMYD5 is a ribosomal methyltransferase that catalyzes RPL40 lysine methylation to enhance translation output and promote hepatocellular carcinoma

This study identifies SMYD5 as a methyltransferase that modifies RPL40 K22me3, enhancing translation and promoting hepatocellular carcinoma.

Single-Cell and Spatial Transcriptomic Analyses Deciphering the Three-Layer Architecture of Human Tuberculosis Granulomas

This study uses single-cell and spatial transcriptomics to analyze human tuberculosis granulomas, revealing a three-layer structure and identifying potential therapeutic targets.

Temporal-spatially metabolic reprogramming rewires the H3K27ac landscape to enable the initiation of liver regeneration

This study identifies metabolic reprogramming and its impact on the H3K27ac epigenetic landscape during liver regeneration.

Microbial single-cell omics in situ

The paper reports a method using LIFT technology to obtain high-quality microbial single-cell genomes or transcriptomes from complex samples and demonstrates its application to study cell-fate commitment in sporulation.

Chemically induced cell plasticity enables the generation of high-fidelity embryo model

This paper develops a chemical-only method to generate high-fidelity embryo models by inducing cell plasticity in stem cells, resulting in embryo-like structures that recapitulate early developmental stages.

Enabling non-viral DNA delivery using lipid nanoparticles co-loaded with endogenous anti-inflammatory lipids

This study develops lipid nanoparticles co-loaded with anti-inflammatory lipids to reduce inflammation from DNA delivery, enabling prolonged transgene expression.

Fe3O4 nanoparticles shell amplify charge-extraction efficiency in Dunaliella photovoltaics

This study enhances Dunaliella-based microbial biophotovoltaics by coating them with Fe3O4 nanoparticles, improving electron transfer efficiency and power output.

Marginated neutrophils in the lungs effectively compete for nanoparticles targeted to the endothelium, serving as a part of the reticuloendothelial system

The paper shows that lung marginated neutrophils effectively uptake endothelial-targeted nanoparticles, suggesting they are part of the reticuloendothelial system.

Ephrin Forward Signaling Controls Interspecies Cell Competition in Pluripotent Stem Cells

This study identifies Ephrin A ligands in mouse cells as mediators of cell death signaling to human pluripotent stem cells during interspecies co-culture, enhancing chimera formation when inhibited.

Molecular Basis of β2 Integrin Activation by Talin Unveils Species-Specific Mechanisms of Integrin Signaling

This study determines the crystal structure of talin head bound to β2-integrin tail, revealing species-specific mechanisms of integrin activation through a two-mode binding configuration.

pH-Controlled chemoselective rapid azo-coupling reaction (CRACR) enables global profiling of serotonylation proteome in cancer cells

The paper develops a chemical proteomic method to globally profile serotonylation in cancer cells, identifying over 1,000 serotonylated proteins and their modification sites.

Enhanced IL-15-mediated NK cell activation and proliferation by an ADAM17 function-blocking antibody involves CD16A, CD137, and accessory cells

This study shows that an ADAM17-blocking antibody enhances IL-15-mediated NK cell activation and proliferation through CD16A and CD137, with potential therapeutic applications in cancer.

Identification and Expression Analysis of CDPK Family in Eriobotrya japonica, reveals EjCDPK25 in Response to Freezing Stress in Fruitlets.

This study identifies and analyzes the CDPK family in loquat, finding EjCDPK25 enhances freezing stress resistance in Arabidopsis.

Curved light sheet microscopy for centimeter-scale cleared tissues imaging

The paper introduces a curved light sheet microscope for high-throughput imaging of cleared tissues, achieving diffraction-limited resolution over a large field of view.

Chemical proteomic profiling of protein dopaminylation in colorectal cancer cells

The paper identifies 425 dopaminylated proteins in colorectal cancer cells using a chemical probe, revealing their involvement in nucleic acid metabolism and transcription, and suggests targeting protein dopaminylation as a potential anti-cancer strategy.

Exogenous myristate fuels the growth of symbiotic arbuscular mycorrhizal fungi but disrupts their carbon-phosphorus exchange with host plants

This study shows that exogenous myristate supports AMF growth but reduces phosphorus transfer to host plants, suggesting it may hinder symbiosis.

Splenic red pulp macrophages eliminate the liver-resistant Streptococcus pneumoniae during bloodstream infection

The paper identifies that splenic red pulp macrophages, aided by natural antibodies and complement, clear liver-resistant Streptococcus pneumoniae from the bloodstream in mice.

Colorectal Cancer Stem Cell Subtypes Orchestrate Distinct Tumor Microenvironments

The paper isolates and characterizes two colorectal cancer stem cell subtypes, showing they create distinct tumor microenvironments and unify existing CRC classifications.

Biochemical and structural insights into a 5' to 3' RNA ligase reveal a potential role in tRNA ligation

This study identifies C12orf29 as an RNA ligase that ligates tRNA and alters tRNA levels in mice, with structural insights into its catalytic mechanism.

Antibody-mediated cellular responses are dysregulated in Multisystem Inflammatory Syndrome in Children (MIS-C)

This study identifies dysregulated antibody-mediated cellular responses in MIS-C, linking NK cell dysfunction to immune pathology and suggesting potential therapeutic interventions.

Lipid Nanoparticle-Associated Inflammation is Triggered by Sensing of Endosomal Damage: Engineering Endosomal Escape without Side Effects

This paper identifies that lipid nanoparticle-induced inflammation arises from endosomal membrane damage and proposes strategies to mitigate it through galectin inhibition or ESCRT-recruiting lipids.

Transcription factor EB (TFEB) interaction with RagC is disrupted during Enterovirus D68 Infection

This paper shows that EV-D68 disrupts TFEB-RagC interaction through 3C protease cleavage, affecting autophagy and viral egress.

Self-organization of vascularized skeletal muscle from bovine embryonic stem cells

This study induces skeletal muscle, neuronal, and endothelial cells from bovine embryonic stem cells and demonstrates their self-organization into vascularized tissue structures.

Bioorthogonal labeling and enrichment of histone monoaminylation reveal its accumulation and regulatory function in cancer cell chromatin

This study develops chemical probes to label and enrich histone monoaminylation, revealing its enrichment in cancer tissues and its role in chromatin architecture regulation.

Evolutionary trajectory of TRPM2 channel activation by adenosine diphosphate ribose and calcium

The paper traces the evolutionary changes in TRPM2 channel activation mechanisms across species, identifying key stages in the transition from simple to complex ligand binding and calcium gating.