M. Goto

Structural basis of eukaryotic transcription termination by the Rat1 exonuclease complex

The paper reports cryo-EM structures of the Rat1-Rai1-Rtt103 complex and RNAPII complexes from yeast, revealing the mechanism of mRNA transcription termination by Rat1 exonuclease.

Exploring the molecular structure of lipids in the design of artificial lipidated antifungal proteins

This study explores lipid modifications of a chitin-binding domain to enhance antifungal activity, finding that specific lipid lengths improve efficacy against Trichoderma viride without significant cytotoxicity.

Engineered Active Zymogen of Microbial Transglutaminase

This paper designs an engineered active zymogen of microbial transglutaminase (EzMTG) that exhibits cross-linking activity without requiring proteolysis, and identifies mutations that balance binding affinity and catalytic activity.

Multi-omics analyses from a single sample: Prior metabolite extraction does not alter the 16S rRNA-based characterization of prokaryotic community in a diversity of sample types

The paper compares 16S rRNA-based prokaryotic community characterization after direct DNA extraction versus metabolite extraction followed by DNA extraction across diverse samples, finding no impact on community composition.

Structural basis of the transcription termination factor Rho engagement with transcribing RNA polymerase

The paper reports the cryo-EM structure of RNA polymerase engaged with Rho, revealing how Rho interacts with RNAP to mediate transcription termination.

Bcr4 is a Chaperone for the Inner Rod Protein in the Bordetella Type III Secretion System

This paper identifies Bcr4 as a chaperone that stabilizes BscI, an inner rod protein of the T3SS, enabling T3SS function in B. bronchiseptica.

Estimation of biodiversity metrics by environmental DNA metabarcoding compared with visual and capture surveys of river fish communities

This study compares eDNA metabarcoding with visual and capture surveys to estimate alpha and gamma diversity in river fish communities, finding eDNA detects more species and provides broader regional insights.

Overexpression of the RNA-binding protein NrdA affects global gene expression and secondary metabolism in Aspergillus species

This study shows that overexpression of NrdA affects global gene expression and secondary metabolism in Aspergillus species, with specific impacts on metabolite production.

Illumina iSeq 100 and MiSeq exhibit similar performance in freshwater fish environmental DNA metabarcoding

The paper compares the performance of Illumina iSeq 100 and MiSeq in fish eDNA metabarcoding, finding similar species detection but differences in species count.

Movements of ancient human endogenous retroviruses detected in SOX2-expressing cells

The paper shows SOX2 regulates HERV-K transcription and retrotransposition in stem cells, suggesting a role in embryonic protection while limiting genomic harm.

LaeA controls citric acid production through regulation of a citrate exporter encoding cexA in Aspergillus luchuensis mut. kawachii

This study identifies LaeA's role in regulating citric acid production in A. kawachii by controlling cexA expression through histone methylation.

HNF1A is a Novel Oncogene and Central Regulator of Pancreatic Cancer Stem Cells

This study identifies HNF1A as a central regulator of pancreatic cancer stem cells and a novel oncogene in pancreatic ductal adenocarcinoma.