A CRISPR-Nonhomologous End-Joining-based strategy for rapid and efficient gene disruption in Mycobacterium abscessus
S. Zeng, Y. Ju, M. S. Alam…·T. Zhang
This paper introduces a CRISPR-NHEJ strategy for efficient gene disruption in Mycobacterium abscessus and shows that homologous recombination and single-strand annealing components are required for NHEJ repair.