C. Fang

Nuclear regulatory disturbances precede and predict the development of Type-2 diabetes in Asian populations

The paper identifies DNA methylation biomarkers predictive of Type-2 diabetes in Asian populations and shows their utility in risk stratification.

MtrAB two-component system is crucial for the intrinsic resistance and virulence of Mycobacterium abscessus

This study shows that disrupting MtrA or MtrB in Mycobacterium abscessus increases antibiotic susceptibility and reduces virulence by affecting cell division and permeability.

Decentralizing genetic testing for biodiversity monitoring and biosurveillance with the Nucleic Acid Barcode Identification Tool (NABIT) and Molecular Development Kit (MDK).

This paper introduces NABIT and MDK for decentralized genetic testing in biodiversity monitoring and biosurveillance, enabling field-ready assays and third-party assay development.

EmbB and EmbC Regulate the Sensitivity of Mycobacterium abscessus to Echinomycin

This study identifies EmbB and EmbC as regulators of Mycobacterium abscessus sensitivity to echinomycin, showing that EmbB mutations can partially compensate for EmbC loss and alter cell wall permeability.

Mutations in ClpC1 or ClpX subunit of the caseinolytic protease confer resistance to natural product ilamycins in mycobacteria

This study identifies two ilamycin analogs targeting mycobacterial Clp protease components and discovers mutations conferring resistance to these compounds.

A CRISPR-Nonhomologous End-Joining-based strategy for rapid and efficient gene disruption in Mycobacterium abscessus

This paper introduces a CRISPR-NHEJ strategy for efficient gene disruption in Mycobacterium abscessus and shows that homologous recombination and single-strand annealing components are required for NHEJ repair.

Deciphering the rule of antigen-antibody amino acid interaction

The paper classifies antigen-antibody amino acid mutations into four types and identifies rules governing immune escape and mutation effects.

Distinct Roles of Kif6 and Kif9 in Mammalian Ciliary Trafficking and Motility

This study identifies distinct roles of Kif6 and Kif9 in mammalian ciliary trafficking and motility, showing Kif6 is a motile cilia-specific IFT motor while Kif9 regulates the central apparatus.

SoyDNGP: A Web-Accessible Deep Learning Framework for Genomic Prediction in Soybean Breeding

SoyDNGP is a deep learning framework for genomic prediction in soybean breeding that outperforms existing methods in accuracy and efficiency, with a web interface for trait prediction and lookup.

High-resolution structure of mouse radial spoke and its in-situ structure in ependymal cilia revealed by cryo-EM and cryo-ET

The paper presents high-resolution cryo-EM structures of mouse radial spokes and cryo-ET maps of ependymal cilia, revealing structural features and mutations linked to ciliary disorders.

TERT accelerates BRAF mutant-induced thyroid cancer dedifferentiation and progression by regulating ribosome biogenesis

This study shows TERT promotes thyroid cancer dedifferentiation and progression by enhancing ribosome biogenesis, and inhibiting rRNA synthesis can reverse this process.

Why is Babesia not killed by artemisinin like Plasmodium?

This study compares Babesia and Plasmodium genomes and gene expression to explain why Babesia is not killed by artemisinin, finding differences in haem synthesis, iron utilization, and gene expression.

Multiple omic investigations of freeze tolerance adaptation in the aquatic ectothermic vertebrate, the Amur sleeper

The paper investigates freeze tolerance in the Amur sleeper using genomic, transcriptomic, and metabolomic analyses to identify genetic and molecular mechanisms underlying this adaptation.

Semi-automated protocol to quantify and characterize fluorescent three-dimensional vascular images

The paper describes a semi-automated protocol to quantify and characterize 3D vascular images in mouse tissues, achieving 80% sensitivity and 90% specificity in automated segmentation.

High-resolution single-molecule long-fragment rRNA gene amplicon sequencing for uncultured bacterial and fungal communities

This study develops a method to sequence nearly full-length rRNA genes from environmental samples, achieving high accuracy and coverage in microbial community analysis.

A recombinant selective drug-resistant M. bovis BCG enhances the bactericidal activity of a second-line tuberculosis regimen

This study develops a drug-resistant recombinant BCG vaccine that enhances second-line TB treatment efficacy in a murine model of drug-resistant tuberculosis.

SARS-CoV-2 RBD-Tetanus toxoid conjugate vaccine induces a strong neutralizing immunity in preclinical studies

Deciphering the mystery of influenza virus A antigenic drift and immune escape

The paper identifies immune escape mutations in influenza A virus HA-tag linear epitopes and proposes establishing an IEMM library to address vaccine challenges.

Breaking the limit of 3D fluorescence microscopy by dual-stage-processing network

This paper introduces a dual-stage deep-learning network to enhance 3D fluorescence microscopy resolution and throughput, achieving faster imaging with improved contrast and subcellular detail.

Efficient and stable metabarcoding sequencing from DNBSEQ-G400 sequencer examined by large fungal community analysis

This study compares the DNBSEQ-G400 and Illumina MiSeq platforms for metabarcoding fungal communities, finding DNBSEQ-G400 to be more accurate, less biased, and cost-effective.

Network-enabled efficient image restoration for 3D microscopy of turbid biological specimens

This paper introduces ScatNet, a deep-learning method for restoring 3D fluorescence microscopy images of turbid biological specimens, improving resolution and signal-to-noise ratio.

Minutes-timescale 3D isotropic imaging of entire organs at subcellular resolution by content-aware compressed-sensing light-sheet microscopy

This paper introduces a computational light-sheet microscopy method that rapidly images entire organs at subcellular resolution, achieving high isotropic spatial resolution and significantly improved throughput.

SARS-COV-2 RBD Oral Vaccine Boosted by Mucosal Immune Adjuvant LTB26 via DCs and B Cells Activation in Mice

This paper develops an oral SARS-COV-2 RBD vaccine using LTB26 adjuvant in mice, showing enhanced antibody production and immune activation through DCs and B cells.

Crl activates transcription by stabilizing the active conformation of the master stress factor σS

This study determines the structure of an E. coli transcription activation complex and shows that Crl stabilizes σS to activate transcription without DNA contact.

Calorie restriction intervention induces enterotype-associated BMI loss in nonobese individuals

This study shows that calorie restriction leads to BMI loss in nonobese individuals, with variations linked to gut microbiota enterotypes.

Distinct gut metagenomics and metaproteomics signatures in prediabetics and treatment-naïve type 2 diabetics

The paper compares gut metagenomics and metaproteomics in prediabetics and treatment-naïve type 2 diabetics to identify host-microbial interplays across disease stages.

Palmitoylation is critically required for cancer intrinsic PD-1 expression and functions

This study identifies PD-1 palmitoylation, its regulatory enzyme, and develops a peptide inhibitor to target PD-1 expression for cancer immunotherapy.

Structural basis for transcription initiation by bacterial ECF σ factors

This study determines the crystal structures of Mycobacterium tuberculosis σH-RNAP and σH-RPo to elucidate the structural basis of ECF σ factor-mediated transcription initiation.

Desiccation Does Not Drastically Increase the Accessibility of Foreign DNA to the Nuclear Genomes: Evidence from the Frequency of Endosymbiotic DNA Transfer

The paper investigates whether desiccation increases foreign DNA integration into nuclear genomes by analyzing NUMT and NUPT abundance in desiccation-tolerant and sensitive organisms, finding no significant increase.

Flavor compounds in fermented Chinese alcoholic beverage alter gut microbiota and attenuate ethanol-induced liver

This study shows that Chinese Baijiu reduces liver injury in mice by altering gut microbiota and metabolome compared to pure ethanol.