Author

R. MacKinnon

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

Click to filter author papers

Biophysics· 8 Biochemistry· 1

Signature Assays, Organisms & Methods

Click to filter · or open tag

Approach:experimental assay· 6 Assay:cryo-EM· 3 Research Focus:cell type annotation· 2 Organism:human· 2 Assay:imaging· 2 Research Focus:protein folding & dynamics· 2 Research Focus:protein structure prediction· 2 Approach:statistical model· 2

Frequent Co-Authors

C. A. Haselwandter· 3 shared G. Vaisey· 2 shared M. E. Falzone· 2 shared X. Tao· 2 shared Y. Guo· 2 shared Z. Fu· 2 shared A. J. North· 1 shared B. Chait· 1 shared

Activity by Year

2017-2024 · peak 4/yr

Co-Author Collaboration Network

10 top collaborators · 12 mutual co-author links

Click any collaborator node to inspect or filter shared papers

Focal author (center)Selected co-authorORCID co-authorUnlinked name

Curved chords = mutual co-authorship

3 shared papersBiophysics

C. A. Haselwandter

Mutual co-authors in cluster

Author profile →

9 papers

Recent·Most cited

FBM: Freestanding bilayer microscope for single-molecule imaging of membrane proteins

G. Perez-Mitta, Y. Sezgin, W. Wang, R. MacKinnon

This paper introduces the Freestanding-Bilayer Microscope (FBM) for single-molecule imaging of membrane proteins and benchmarks it against TIRF imaging to study ion channel dynamics.

bioRxiv · Jan 26, 2024Biophysics

The mechanism of Gαq regulation of PLCβ3-catalyzed PIP2 hydrolysis.

M. E. Falzone, R. MacKinnon

This paper shows Gq increases PLCβ3 catalytic rate and structures reveal G proteins regulate PLCβ3 via separate mechanisms, leading to a product rule of co-stimulation.

bioRxiv · Aug 29, 2023 · PreprintBiophysics

Gβγ Activates PIP2 Hydrolysis by Recruiting and Orienting PLCβ on the Membrane Surface

M. E. Falzone, R. MacKinnon

This study shows Gβγ activates PLCβ3 by recruiting and orienting it on the membrane, increasing catalytic efficiency and reducing background activity.

bioRxiv · Dec 20, 2022 · PreprintBiophysics

Piezo1 as a force-through-membrane sensor in red blood cells.

G. Vaisey, P. Banerjee, A. J. North, C. A. Haselwandter, R. MacKinnon

This study investigates Piezo1 distribution and dynamics in red blood cells, finding it preferentially localizes to the dimple and exhibits curvature coupling behavior.

bioRxiv · Aug 10, 2022Biochemistry

Elastic properties and shape of the Piezo dome underlying its mechanosensory function

C. A. Haselwandter, Y. Guo, Z. Fu, R. MacKinnon

The paper determines the force-distortion relationship of Piezo channels using vesicle shape measurements and derives their intrinsic curvature and bending stiffness.

bioRxiv · Jul 1, 2022Biophysics

Quantitative prediction and measurement of Piezo's membrane footprint

C. A. Haselwandter, Y. Guo, Z. Fu, R. MacKinnon

The paper predicts and measures the membrane footprint of Piezo proteins using elasticity theory and cryo-electron tomography, showing agreement across vesicle sizes and revealing a spherical cap geometry.

bioRxiv · Jun 28, 2022Biophysics

Analysis of the Mechanosensor Channel Functionality of TACAN

Y. Niu, X. Tao, G. Vaisey, P. D. B. Olinares, H. Alwaseem, B. Chait, R. MacKinnon

The paper investigates TACAN's functionality as a mechanosensitive ion channel and finds it does not exhibit mechanosensitive activity, instead showing structural features related to fatty acid elongase.

bioRxiv · Jun 11, 2021Biophysics

Correlation between structure and function in phosphatidylinositol lipid-dependent Kir2.2 gating

R. MacKinnon, Y. Zhang, X. Tao

The paper investigates how phosphatidylinositol lipids, particularly PI(4,5)P2, affect Kir2.2 channel gating by analyzing burst and inter-burst durations in planar lipid bilayers.

bioRxiv · Feb 15, 2021Biophysics

Molecular structure of human KATP in complex with ATP and ADP

K. Lee, J. Chen, R. MacKinnon

The paper presents two cryo-EM structures of the human KATP channel complexed with ATP and ADP, revealing their roles in channel regulation.

bioRxiv · Oct 12, 2017Biophysics