S. Barg

Molecular mapping and functional validation of GLP-1R cholesterol binding sites in pancreatic beta cells

This study identifies and validates cholesterol binding sites on GLP-1R in pancreatic beta cells, showing their role in receptor function and potential for drug design.

RFX6 haploinsufficiency predisposes to diabetes through impaired beta cell functionality

This study shows that RFX6 haploinsufficiency impairs beta cell function, leading to reduced insulin secretion and increased diabetes risk.

Real-time TIRF imaging of single adiponectin vesicle exocytosis in 3T3-L1 adipocytes stably expressing mCherry fused to human adiponectin

This study uses TIRF microscopy to observe and quantify single adiponectin vesicle exocytosis in 3T3-L1 adipocytes expressing mCherry-adiponectin, showing that both cAMP and Ca2+ elevation trigger release.

Restoration of PITPNA in Type 2 diabetic human islets reverses pancreatic beta-cell dysfunction

The paper shows restoring PITPNA in T2D human islets reverses beta-cell dysfunction by improving insulin granule maturation and glucose-stimulated insulin secretion.

Phase Separation of a Nodulin Sec14-like protein Maintains Auxin Efflux Carrier Polarity at Arabidopsis Plasma Membranes

The paper shows that phase separation of SEC14-like protein, mediated by proteolysis, maintains auxin efflux carrier polarity at Arabidopsis plasma membranes, contributing to root developmental robustness.

Local PI(4,5)P2 generation controls fusion pore expansion during exocytosis

The paper shows that local PI(4,5)P2 signaling controls fusion pore expansion during exocytosis by recruiting endocytic machinery and preventing peptide release.

The β-cell primary cilium is an autonomous Ca2+ compartment for paracrine GABA signalling

The paper investigates Ca2+ dynamics in β-cell primary cilia and identifies GABA-B1 receptor-mediated paracrine signaling as a localized Ca2+ signaling mechanism.

Paracrine control of α-cell glucagon exocytosis is compromised in human type-2 diabetes

This study shows that paracrine inhibition of α-cell glucagon exocytosis is impaired in human type-2 diabetes, leading to elevated glucagon levels.

Fusion pore regulation by Epac2/cAMP controls cargo release during insulin exocytosis

This study shows that Epac2/cAMP regulates fusion pore expansion in insulin exocytosis, affecting hormone and transmitter release balance.