S. P. S. Chundawat

This study examines how galactose and manganese supplementation affects Trastuzumab N-glycosylation over time in fed-batch and perfusion bioreactors, showing temporal changes in glycoform composition.

This paper develops a method to isolate and characterize plant cellulose synthase enzymes from Pichia pastoris protoplasts, achieving higher yields and functional reconstitution into liposomes.

This paper introduces a method to create bead-based avidity model systems using effector beads for measuring avidity effects in cell-cell interactions and drug potency assessment.

This study engineers and characterizes tandem carbohydrate-binding modules (CBMs) to enable real-time imaging of cellulose fibril biosynthesis in Arabidopsis protoplasts.

The paper predicts binding energy for combinatorial mutants of an alpha-L-fucosidase using machine learning trained on docking data.

The paper develops a MATLAB simulation package for tethered particle motion (TPM) to model and visualize biophysical systems, validated against experimental techniques like Acoustic Force Spectroscopy.

The paper develops an automated PAT system called N-GLYcanyzer for rapid monitoring of mAb N-linked glycosylation during biomanufacturing.

This study shows that supercharging cellulase surface charge improves hydrolytic activity on pretreated biomass by reducing non-productive binding.

This paper uses acoustic force spectroscopy to study how carbohydrate-binding modules (CBMs) unbind from nanocellulose surfaces, revealing distinct binding conformations and their impact on cellulolytic activity.

The paper investigates how reducing carbohydrate-binding module (CBM) interactions with cellulose improves endocellulase activity by analyzing binding kinetics and hydrolysis efficiency.

The paper compares four analytical methods for quantifying soluble carbohydrates in lignocellulosic biomass and provides an educational protocol for bioenergy teaching.

This study uses single-molecule assays and simulations to show how CBM1 binding affects cellulase activity on different cellulose allomorphs, highlighting limitations of classical binding models.

This study shows carbohydrate-binding domains enhance catalytic activity in chimeric CAZymes for efficient oligosaccharide synthesis.

This paper develops a click-chemistry-based method for high-throughput screening of glycosynthase mutants to synthesize fucosylated oligosaccharides.