S. Pfeffer

DEAD box RNA helicase 5 is a new pro-viral host factor for Sindbis virus infection

This study identifies DDX5 as a pro-viral host factor for Sindbis virus infection, showing its interaction with viral RNA and capsid protein, and demonstrating that its depletion reduces viral replication.

Mitochondrial dysfunction rapidly modulates the abundance and thermal stability of cellular proteins

The paper develops ppTPP to study mitochondrial dysfunction's effects on proteome stability and abundance, revealing group-specific response patterns in eukaryotic cells.

Translational Activity Controls Ribophagic Flux and Turnover of Distinct Ribosome Pools

The paper investigates ribosome turnover in human cells, showing distinct subunit pools with different turnover rates and demonstrating that translation inhibition increases 80S ribosome pool size and induces ribophagy.

RACK1 associates with RNA-binding proteins Vigilin and SERBP1 to control dengue virus replication

This study identifies RACK1 as a host factor that recruits Vigilin and SERBP1 to facilitate dengue virus replication by linking viral RNA to the translation machinery.

Human DICER helicase domain recruits PKR and modulates its antiviral activity

The paper shows that DICER's helicase domain recruits PKR and modulates its antiviral activity during alphavirus infection.

Proteomics-based determination of double stranded RNA interactome reveals known and new factors involved in Sindbis virus infection

This study identifies SFPQ as a new proviral factor involved in Sindbis virus infection by characterizing the dsRNA interactome using anti-dsRNA immunoprecipitation and mass spectrometry.

Genome-wide CRISPR-Cas9 screen reveals the importance of the heparan sulfate pathway and the conserved oligomeric golgi complex for synthetic dsRNA uptake and Sindbis virus infection

This study identifies the heparan sulfate pathway and COG4 in the Conserved Oligomeric Golgi complex as critical for dsRNA uptake and Sindbis virus infection in human cells.

Template-free detection and classification of heterogeneous membrane-bound complexes in cryo-electron tomograms

The paper develops a template-free method to detect and classify membrane-bound complexes in cryo-ET data, revealing previously undetected ER translocon species and their structural and spatial characteristics.

mTORC1 controls phase-separation and the biophysical properties of the cytoplasm by tuning crowding

The paper investigates how mTORC1 regulates macromolecular crowding and biophysical properties of the cytoplasm by using GEMs to measure diffusion and phase-separation changes.