G. Kramer

Non-mechanical disruption of Bacillus subtilis spores allows sensitive and deep characterization of the minimal proteome for resuming a cellular lifestyle.

This paper introduces a new sample preparation method, SPEED, which improves proteomic analysis of Bacillus subtilis spores by identifying more proteins and enabling differentiation between spore and vegetative cell proteins.

Isolation and characterization of persisters of the pathogenic microorganism Staphylococcus aureus

This study isolates and characterizes persisters of Staphylococcus aureus induced by vancomycin and enrofloxacin, revealing molecular and metabolic differences and factors affecting their formation.

Multi-omics analysis of Bacillus. subtilis spores formed at different environmental temperatures reveal differences at the morphological and molecular level.

This study analyzes B. subtilis spores formed at different temperatures to identify molecular and morphological differences.

Molecular physiological characterization of the dynamics of persister formation in Staphylococcus aureus.

This study investigates the molecular dynamics of persister formation in Staphylococcus aureus under vancomycin and enrofloxacin exposure, revealing stress-specific molecular profiles and the potential of combined therapies.

Integrative metabolomics and proteomics allow the global intracellular characterization of Bacillus subtilis cells and spores.

This study uses metabolomics and proteomics to characterize B. subtilis vegetative cells and spores, showing differences between them and demonstrating the effectiveness of the 60% EtOH extraction protocol.

Whitefly effector G4 interacts with tomato proteins of which MIPDB141 affects whitefly performance

The paper identifies effector G4 from whitefly Bemisia tabaci and shows it suppresses tomato immunity by interacting with MIPDB141, reducing whitefly fecundity.

Integrated post-genomic cell wall analysis reveals floating biofilm formation associated with high expression of flocculins in the pathogen Candida krusei.

This study characterizes the cell wall of Candida krusei, identifying its proteome and showing that static cultures form floating biofilms with increased flocculin expression.

Cross-link scrambling in peptide pairs

The paper identifies cross-link types in peptides using SCXC retention time prediction and shows that MS/MS analysis reveals ester linkages in some amide-predicted pairs due to gas-phase rearrangement.

Translational Activity Controls Ribophagic Flux and Turnover of Distinct Ribosome Pools

The paper investigates ribosome turnover in human cells, showing distinct subunit pools with different turnover rates and demonstrating that translation inhibition increases 80S ribosome pool size and induces ribophagy.

Genetic elements orchestrating Lactobacillus crispatus glycogen metabolism in the vagina

This study identifies genetic variation and regulation of the pulA gene in Lactobacillus crispatus, showing its role in glycogen metabolism and its presence in vaginal microbial communities.

Direct observation of Hsp90-induced compaction in a protein chain

This study uses optical tweezers to show Hsp90 induces local contractions in unfolded protein chains, leading to global compaction and suppression of misfolding and aggregation.

High resolution analysis of proteome dynamics during Bacillus subtilis sporulation

This study uses high-resolution time-lapse proteomics to analyze proteome dynamics during Bacillus subtilis sporulation, identifying co-expressed modules and potential new sporulation-related proteins.

A composite filter for low FDR of protein-protein interactions detected by in vivo cross-linking

The paper introduces a composite filter to reduce the false discovery rate of protein-protein interactions detected by in vivo cross-linking using BAMG and pLink 2.

The Ice Nucleating Protein InaZ is Activated by Low Temperature

This study investigates how the ice-nucleating protein InaZ from Pseudomonas syringae folds and interacts with water at low temperatures, revealing structural changes that enhance ice nucleation.

Thyroid and androgen receptor signaling are antagonized by CRYM in prostate cancer

This study shows that CRYM antagonizes thyroid and androgen signaling in prostate cancer and correlates with prognosis and PET imaging biomarkers.

The C-terminal tail of the bacterial translocation ATPase SecA modulates its activity

This study investigates how the C-terminal tail of SecA modulates its activity in cotranslational substrate protein recognition by interacting with ribosomes and substrate proteins.