F. Stein

Trifunctional fatty acid derivatives demonstrate the impact of diazirine placement

The paper synthesizes three palmitic acid derivatives with diazirine at different positions and shows that end-position diazirines crosslink distinct protein subsets.

Revealing the Arabidopsis AtGRP7 mRNA binding proteome by specific enhanced RNA interactome capture

The paper identifies proteins interacting with the AtGRP7 mRNA in Arabidopsis using enhanced RNA interactome capture, revealing ALBA proteins as binders.

Small molecule modulation of a redox-sensitive stress granule protein dissolves stress granules with beneficial outcomes for familial amyotrophic lateral sclerosis models

The paper identifies lipoamide as a small molecule that dissolves stress granules and improves outcomes in ALS models by modulating redox-sensitive proteins.

Trifunctional sphinganine: a new tool to dissect sphingolipid function

The paper develops multifunctional derivatives of sphingosine and sphinganine to study their cellular roles, revealing distinct protein interactomes and spatiotemporal distributions.

Global RNA interactome of nitrogen starved Escherichia coli uncovers a conserved post-transcriptional regulatory axis required for optimal growth recovery

This study maps the Hfq-mediated RNA interactome in nitrogen-starved E. coli, revealing SdsR's role in post-transcriptional regulation during long-term growth arrest and recovery.

A ubiquitin-specific, proximity-based labeling approach for the identification of ubiquitin ligase substrates

The paper introduces a proximity-based labeling method to identify ubiquitin ligase substrates and validates it using RAD18, TRAF6, and CHIP.

Flow in fetoplacental microvessels in vitro enhances perfusion, barrier function, and matrix stability

This study develops a 3D in vitro model of human placental microvessels and shows that flow enhances perfusion, barrier function, and matrix stability.

Dextromethorphan inhibits collagen transport in the endoplasmic reticulum eliciting an anti-fibrotic response in ex-vivo and in vitro models of pulmonary fibrosis

This study shows Dextromethorphan reduces collagen deposition in fibrotic models by inhibiting collagen trafficking in the endoplasmic reticulum.

Inter-laboratory comparison of plant volatile analyses in the light of intra-specific chemodiversity

This study assesses the reproducibility of plant volatile organic compound (VOC) analysis across five laboratories using Tanacetum vulgare, finding high variation in VOC profiles and JA-induced changes.

Mitochondrial dysfunction rapidly modulates the abundance and thermal stability of cellular proteins

The paper develops ppTPP to study mitochondrial dysfunction's effects on proteome stability and abundance, revealing group-specific response patterns in eukaryotic cells.

A new technology for isolating organellar membranes provides fingerprints of lipid bilayer stress

The paper introduces a method to isolate organellar membranes from yeast and uses it to study lipid composition and stress responses in the endoplasmic reticulum and vacuolar membranes.

Lysosome-targeted lipid probes reveal sterol transporters NPC1 and LIMP2 as sphingosine transporters

The paper develops lipid probes to study sphingosine transport, revealing NPC1 and LIMP2 as sphingosine transporters and showing their role in cholesterol efflux.

Increased levels of the mitochondrial import factor Mia40 prevent the aggregation of polyQ proteins in the cytosol

The paper shows that overexpressing Mia40 reduces toxic polyQ protein aggregates in yeast and human cells by competing for chaperone and proteasome resources.

Pharmacological inhibition of nonsense-mediated RNA decay augments HLA class I-mediated presentation of neoepitopes in MSI CRC

The paper shows that inhibiting nonsense-mediated RNA decay increases HLA class I presentation of neoepitopes from frameshift mutations in MSI colorectal cancer.

Bacterial retrons encode tripartite toxin/antitoxin systems

The paper identifies retrons as a novel family of tripartite toxin/antitoxin systems in bacteria, with the reverse transcriptase and msDNA functioning as antitoxin components.

Global mapping of Salmonella enterica-host protein-protein interactions during infection

The paper maps Salmonella enterica effector-host protein-protein interactions during infection, identifying 421 novel interactions and demonstrating their roles in pathogenesis.

Stroma transcriptomic and proteomic profile of prostate cancer metastasis xenograft models reveals conservation of bonemicroenvironment signatures

The paper identifies tenascin C as a stromal marker associated with metastasis in prostate cancer xenograft models and shows its prognostic potential in patient samples.

Multiomic and quantitative label-free microscopy-based analysis of ex vivo culture and TGFbeta1 stimulation of human precision-cut lung slices

The paper uses multiomic and microscopy techniques to study fibrosis in human lung slices, showing increased collagen deposition with TGFbeta1 stimulation and a novel SHG-based method to quantify it.

Systematic localization of Gram-negative bacterial membrane proteins

This study systematically maps membrane protein localization in E. coli using mass spectrometry, identifying discrepancies with existing annotations and providing a reference dataset for Gram-negative bacteria.

The outer membrane lipoprotein NlpI nucleates hydrolases within peptidoglycan multi-enzyme complexes in Escherichia coli

This study identifies NlpI as a scaffold for PG hydrolases in E. coli, forming multi-enzyme complexes that coordinate PG synthesis and hydrolase activity.

Effect of Sec61 interaction with Mpd1 on Endoplasmic Reticulum-Associated Degradation

The paper identifies Mpd1 as an ERAD factor that interacts with Sec61 and shows that this interaction is required for CPY* ERAD.