M. Dogterom

Dynamic instability of force-generating bacterial microtubules

The paper investigates dynamic instability in bacterial microtubules of Prosthecobacter, showing similar behavior to eukaryotic microtubules and revealing structural features like doublets.

Stable kinetochore-microtubule attachment requires loop-dependent Ndc80-Ndc80 binding

This study shows that the Ndc80 loop facilitates Ndc80-Ndc80 interactions, which are essential for stable kinetochore-microtubule attachments and proper chromosome congression.

CSPP1 stabilizes growing microtubule ends and damaged lattices from the luminal side.

This study identifies CSPP1 as a microtubule stabilizer that binds to growing and damaged microtubules, providing insights into its role in ciliary and centriolar structure maintenance.

Estimation of microtubule-generated forces using a DNA origami nanospring

This paper introduces a DNA origami nanospring to estimate microtubule-generated forces using fluorescence microscopy and validates it against optical trapping.

Strain stiffening of Ndc80 complexes attached to microtubule plus ends

The paper measures strain stiffening of Ndc80 complexes under tension from depolymerizing microtubules, showing it is robust to mutations and linked to structural features and thermal fluctuations.

Microtubule plus-end regulation by centriolar cap proteins

The paper investigates how CP110 and CEP97 regulate microtubule plus-end dynamics and centriole biogenesis through direct interactions and synergistic effects.

EB3-informed dynamics of the microtubule stabilizing cap during stalled growth

The paper investigates microtubule stability dynamics using EB3 as a marker, showing a simple model explains catastrophe rates and stalled growth behavior.

Multivalent interactions facilitate motor-dependent protein accumulation at growing microtubule plus ends

The paper shows that multivalent interactions enable motor-dependent protein accumulation at microtubule ends through disordered networks and liquid droplet formation in a reconstituted fission yeast system.

Cross-linkers at growing microtubule ends generate forces that drive actin transport

This paper shows that passive cross-linkers between actin and growing microtubules generate forces that transport actin filaments, revealing a new mechanism for actin remodeling.

Non-spherical coacervate shapes in an enzyme driven active system

This study investigates how enzymatic activity influences the shape and stability of RNA coacervates, finding that PNPase affects morphology and degradation.

Reconstitution of basic mitotic spindles in cell-like confinement

This study reconstitutes basic mitotic spindles in cell-like confinement using emulsion droplets, revealing mechanisms of spindle organization and force balance.

Motor-mediated clustering at microtubule plus ends facilitates protein transfer to a bio-mimetic cortex

The paper investigates how motor-mediated clustering at microtubule plus ends facilitates protein transfer to a bio-mimetic cortex, showing that clustered proteins transfer more effectively than non-clustered ones.

Mechanisms of motor-independent membrane remodeling driven by dynamic microtubules

This study reconstitutes membrane tubulation using dynamic microtubules and proteins, revealing three motor-independent mechanisms for membrane remodeling.

CRISPR-based DNA and RNA detection with liquid-liquid phase separation

This paper introduces a label-free CRISPR-based method for nucleic acid detection using liquid-liquid phase separation, showing that target sequence cleavage reduces turbidity.

NDC80 clustering modulates microtubule dynamics under force

The paper shows that NDC80 clustering increases microtubule binding affinity and enables force-dependent modulation of kinetochore-microtubule attachments.