One-step generation of auxin-inducible degron cells with high-efficiency homozygous tagging
S. Li, Y. Wang, M. v. d. Stoel…·E. Ikonen
This paper introduces a one-step genome editing method for high-efficiency homozygous tagging of endogenous proteins with auxin-inducible degron in human cells, achieving >80% efficiency in A431 cells and >50% in H9 ES cells.