Wen-Hsin Lee

This study tracks germinal center responses in rhesus macaques over a year, showing prolonged GCs with declining SHM and ongoing plasma cell production.

This study identifies potent antimalarial antibodies in African infants vaccinated with RTS,S/AS01E, showing near-germline configurations and protective efficacy.

This paper develops a consensus HCV E1E2 antigen that engages germline B cell precursors of broadly neutralizing antibodies.

Epitope-focused S2 stem-helix nanoparticle immunogens elicit protective broadly neutralizing antibodies against diverse betacoronaviruses in non-human primates, establishing a framework for pan-betacoronavirus vaccines.

This paper describes the development and cGMP manufacturing of an HIV vaccine candidate for clinical trials, demonstrating scalable production and quality control.

This study determines the structural basis of antigen recognition by two mAbs targeting PfCSP, revealing conserved and distinct binding modes and their implications for antibody function.

This study uses deep learning to design multi-epitope scaffolds mimicking CSP's spatial structure, showing accurate positioning of two epitopes and partial success with the third.

This study shows that a germline-targeting HIV immunogen induces cross-neutralizing antibodies in outbred macaques by priming V2-apex bnAb B cell precursors.

The paper designs native-like HIV Env single-chain trimers (SCTs) that are stable, immunogenic, and compatible with vaccine delivery methods.

Membrane-bound BG505 MD39.3 Env trimer delivered as mRNA-LNP reduced base-directed antibody responses in rabbits and macaques and elicited neutralizing antibodies, with memory B cell responses documented in macaques.

The paper presents a nanodisc platform for assembling transmembrane glycoproteins into vaccine candidates, demonstrating its utility in HIV vaccine development through structural and functional analyses.

The paper identifies two human monoclonal antibodies that broadly inhibit PfEMP1's binding to EPCR, a key process in severe malaria pathogenesis.

This study immunized cows with HIV Env trimers to generate broadly neutralizing antibodies targeting the V2-apex, revealing ultralong CDRH3 antibodies outcompete shorter ones in the response.

This study identifies neutralizing antibody epitopes against Lassa virus GPC using electron microscopy, revealing challenges in vaccine-induced responses and opportunities for improved vaccine design.

The paper introduces a triple tandem trimer (TTT) design for HIV-1 Env and influenza HA immunogens, showing improved trimer-specific immune responses in viral vectored vaccines.

This study investigates whether engineering HIV Env trimers or using gp120 can induce broadly neutralizing antibodies in cows, finding that trimers are more effective than monomeric gp120.

The paper determines the structure of Arabidopsis thaliana OSCA2.3 using cryo-EM, revealing conformational heterogeneity and a conserved interaction between the TM6a-TM7 linker and the Beam-Like Domain.

The paper identifies structural elements in OSCA channels that differentially mediate stretch and poking activation through mutagenesis and electrophysiology.

This study shows that germline-targeting SCIVs can activate and expand B cells expressing V2-apex bnAb-like precursors in rhesus macaques.