Jeetain Mittal

The paper introduces a computational framework to model pH gradients in biomolecular condensates and shows sequence-dependent pH shifts in FUS and PGL-3.

This study shows that scaffold binding affinity determines condensate phase behavior, dynamics, and biochemical output in vitro and in human cells.

The paper investigates how condensate material properties affect exopher cargo selection in neurons, finding that gel-like condensates are more efficiently recruited than liquid-like ones.

This study investigates how electrostatic interactions influence HP1α phase separation and its interactions with binding partners, showing that these interactions modulate HP1's properties and functions.

The paper investigates how biomolecular condensates alter amino acid pKa values through simulations, finding protonated states are favored in condensates.

This paper develops a graph-based model to predict small-molecule partitioning into biomolecular condensates using existing experimental data and applies it to identify chemically diverse partitioners.

The paper shows that endosome maturation is regulated by proton signaling through NHE6 and pH-dependent Rab7 cycling, with implications for neurodegeneration.

Short UG-rich RNAs bind and stabilize TDP-43 RNA-recognition motifs to allosterically destabilize its prion-like domain helical region, preventing aggregation and mitigating neurodegeneration in cells and mice.

The paper investigates how mitochondrial replication components form multiphasic condensates and shows how ssDNA and dsDNA are segregated by architectural proteins.

This study introduces β-sheet breaking proline residues into FUS prion-like domain to prevent aggregation and toxicity while preserving phase separation and function.

This study develops a flow cytometry method to measure biomolecular condensate formation, colocalization, and exchange dynamics, revealing that condensate aging reduces molecular dynamics.

This study uses molecular dynamics simulations to show that sequence-dependent electrostatic interactions govern condensate viscosity and diffusivity in IDPs.

This paper investigates how the disordered C-terminal domain of TDP-43 interacts with RNA and identifies key residues and structural features that mediate RNA recognition and binding specificity.

This study shows methionine oxidation in TDP-43's C-terminal domain disrupts structure and phase separation through region-specific mechanisms.

This study shows RNA modulates FUS condensate assembly and aggregation through specific molecular interactions, affecting phase separation and aggregate formation.

This study investigates the structural basis of TDP-43 C-terminal domain multimerization using biophysical experiments, AlphaFold2-Multimer, and simulations, revealing helical oligomerization and the role of specific residues in phase separation and disease.

This study uses multiscale simulations to investigate polyglutamine aggregation mechanisms and how the N17 flanking domain influences fibril polymorphism in Huntingtons disease.

This paper investigates how EWS's RNA-binding domains interact with its low-complexity domain to influence condensate formation, revealing electrostatic and residue-specific interactions that underlie EWS self-association.

This study uses a coarse-grained model to show DNA length and flexibility influence protein-DNA condensate structure and protein dynamics, revealing multiphasic organization and differential diffusion modes.

This study uses atomistic simulations to analyze the conformational dynamics and interdomain interactions of full-length FUS during phase separation, revealing key electrostatic interactions and domain stability effects.

This study uses simulations to show how small-molecule cosolutes alter condensate morphology by changing interaction strengths and stoichiometry.

This study calculates amino acid transfer free energies to reveal thermodynamic contributions to biomolecular condensate formation, showing charge asymmetry and interactions between protein and water.

This study investigates how heterotypic protein interactions affect α-synuclein condensate dynamics and aggregation, showing that multivalent electrostatic interactions drive co-condensation and promote amyloid fibril formation.

This study shows that TDP-43 aggregation in stress granules requires up-concentration and oxidative stress, leading to intra-condensate demixing and pathological aggregates.

This study investigates the molecular interactions driving phase separation in MBD2 and MBD3 proteins, revealing distinct mechanisms despite their structural similarity.

This study uses molecular dynamics simulations to show that transient interdomain interactions in Httex1 modulate its structural ensemble and self-assembly, contributing to aggregation in Huntington's disease.

This paper characterizes the structure and dynamics of HSPB1 oligomers using biophysical and computational methods, revealing conformational heterogeneity and domain-specific interactions.

The paper expands the molecular grammar of polar residues and arginine in FUS prion-like domain phase separation and aggregation by showing additional residues contribute to these processes.

This paper introduces a computational microrheology technique to quantify sequence-dependent viscoelasticity of protein condensates formed by intrinsically disordered proteins.

This study uses molecular dynamics simulations to show RNA-binding stabilizes TDP-43 tandem RRMs and reduces conformational plasticity, preventing pathological aggregation.