W. Chen

Individualized and stereotypical seizure semiology in a porcine model of post-traumatic epilepsy.

This study examines seizure patterns in a porcine model of post-traumatic epilepsy, finding that seizures start focally and evolve to generalized convulsions with stereotypical behaviors.

Intracellular lipopolysaccharide binds RETREG1/FAM134B to regulate ER remodeling upon bacterial infection

This study shows that bacterial LPS binds RETREG1 to induce ER remodeling and enhance antibacterial defense through ERphagy.

Evolutionary genomics of local adaptation, climate vulnerability, and linarin metabolism in Opisthopappus longilobus

This study presents the first chromosome-scale genome of Opisthopappus longilobus, identifies adaptive genes linked to climate stress, and links genetic divergence to metabolic differences between species.

Scalable Generation of Clinical-Grade Universal Human cDC1s Enables Potent Antitumor Immunotherapy

This paper develops a scalable method to generate clinical-grade cDC1s from umbilical cord blood, showing improved antitumor efficacy in mouse models and recapitulating DC differentiation.

Spatiotemporal transcriptomic profiling reveals the dynamic immunological landscape of alveolar echinococcosis

This study uses spatiotemporal transcriptomics to analyze immune dynamics in E. multilocularis infected mouse liver, identifying key immune cell roles and shifts in immune strategy during infection.

Design of intrinsically disordered region binding proteins

The paper designs proteins that bind intrinsically disordered regions with high affinity and specificity across diverse targets.

BOOST: a robust ten-fold expansion method on hour-scale

BOOST is a rapid expansion microscopy method that achieves ~10x linear expansion in 90 minutes using microwave-assisted chemistry, improving throughput for various biological specimens.

Genomic characterization of Escherichia coli harbor a polyketide synthase (pks) island associated with colorectal cancer (CRC) development

This study characterizes the genomic distribution and evolution of pks islands in E. coli strains associated with colorectal cancer, revealing phylogenetic patterns and horizontal gene transfer.

Short heat shock factor A2 confers heat sensitivity in Arabidopsis: Insights into heat resistance and growth balance

This study identifies S-HsfA2 in Arabidopsis as a factor that prevents hyperactivation of the heat stress response, balancing heat resistance and growth.

CAMSAP3 Forms Dimers via its lapha-helix Domain that Directly Stabilize Non-centrosomal Microtubule Minus Ends

This study identifies how CAMSAP3 dimerizes via its C-terminal alpha-helix to stabilize non-centrosomal microtubule minus ends through enhanced CKK domain binding.

Nuclear phylogenomics of grasses (Poaceae) supports current classification and reveals repeated reticulation

This study constructs a nuclear phylogeny of grasses using genome-scale data, showing agreement with plastome phylogeny and evidence for repeated reticulation events.

Protein Structure Inspired Drug Discovery

The paper uses protein 3D structure analysis to discover novel anticancer compounds by targeting unique structural motifs, identifying Dxr2-017 as a selective inhibitor of melanoma cells.

Delta-5 elongase knockout reduces DHA and TAG synthesis coupled with an increase of heat sensitivity in a marine diatom

This study shows that knocking out delta-5 elongase in a marine diatom reduces DHA and TAG synthesis and increases heat sensitivity.

Carbon Dioxide Controls Fungal Fitness and Skin Tropism of Candida auris

This study identifies a carbonic sensing pathway (CSP) in Candida auris that enhances drug resistance and skin colonization through mechanisms involving ROS response, cell wall remodeling, and energy metabolism.

Metabolically intact nuclei are fluidized by the activity of the chromatin remodeling motor BRG1

This study shows that BRG1 activity fluidizes the nucleus, altering its stiffness and dissipating mechanical energy, with implications for nuclear mechanics and remodeling.

DNA-based ForceChrono Probes for Deciphering Single-Molecule Force Dynamics in Living Cells

This paper introduces DNA-based ForceChrono probes to measure single-molecule force dynamics in living cells, revealing precise loading rates and a link between force magnitude and duration.

Circular RNA HMGCS1 sponges miR4521 to aggravate type 2 diabetes-induced vascular endothelial dysfunction

This study identifies circHMGCS1 as a regulator of vascular endothelial dysfunction in diabetes by sponging miR4521, leading to impaired nitric oxide production and increased oxidative stress.

ATG6 interacting with NPR1 increases Arabidopsis thaliana resistance to Pst DC3000/avrRps4 by increasing its nuclear accumulation and stability

This study shows ATG6 interacts with NPR1, enhancing its nuclear accumulation and stability, which boosts Arabidopsis resistance to Pst DC3000/avrRps4.

Study of Impacts of Two Types of Cellular Aging on the Yeast Bud Morphogenesis

This study analyzes two aging modes in yeast, showing spherical budding cells better control growth and size, and develops a model to test mechanisms of bud morphogenesis during aging.

Enhanced microbiota profiling in patients with quiescent Crohn's disease through comparison with paired healthy first-degree relatives using fecal metagenomics and metabolomics

The paper compares microbiota and metabolome profiles of quiescent Crohn's disease patients with their healthy first-degree relatives to identify biomarkers associated with disease relapse.

The impact of Bacillus coagulans X3 on available nitrogen content, bacterial community composition, and nitrogen functional gene levels when composting cattle manure

This study examines how adding Bacillus coagulans X3 to cattle manure compost affects nitrogen levels, bacterial communities, and nitrogen-related genes.

A Vascular Dissection and Rupture Linked Metabolite Acts Via BLT2 Receptor

This study identifies 12-HETE as a key metabolite in thoracic aortic dissection (TAD) that activates BLT2 receptors on macrophages, driving inflammation and VSMC phenotype switching, and shows that targeting this pathway can reduce TAD progression.

Ca2+-sensor ALG-2 engages ESCRTs to enhance lysosomal membrane resilience to osmotic stress

The paper shows that ALG-2, a Ca2+ sensor, recruits ESCRTs to lysosomes to enhance their resilience against osmotic stress-induced rupture.

Modeling platinum resistance in a stem-like patient-derived ovarian cancer sample

This study develops a patient-derived model of cisplatin resistance in ovarian cancer stem-like cells and identifies molecular and functional changes associated with resistance.

Dissecting reprogramming heterogeneity at single-cell resolution using scTF-seq

The paper introduces scTF-seq, a method to study TF dose-dependent reprogramming in mouse MSCs, revealing heterogeneity in cell fate and TF sensitivity.

Self-inspired learning to denoise for live-cell super-resolution microscopy

This paper introduces SN2N, a self-supervised denoising method for live-cell super-resolution microscopy that achieves competitive performance without requiring large clean datasets.

Cbfβ regulates Wnt/β-catenin, Hippo/Yap, and TGFβ signaling pathways in articular cartilage homeostasis and protects from ACLT surgery-induced osteoarthritis

This study shows that Cbfβ deficiency leads to OA by altering Wnt/β-catenin, Hippo/Yap, and TGFβ signaling, and that local Cbfβ overexpression protects against OA in mice.

Blocker-SELEX: A Structure-guided Strategy for Developing Inhibitory Aptamers Disrupting Undruggable Transcription Factor Interactions

This paper introduces Blocker-SELEX, a structure-guided method to design inhibitory aptamers that disrupt transcription factor interactions, demonstrating efficacy against SCAF4/SCAF8-RNAP2 and WDR5-MYC complexes.

An infection and pathogenesis mouse model of SARS-CoV-2-related pangolin coronavirus GX_P2V(short_3UTR)

This study establishes a mouse model using GX_P2V(short_3UTR) to evaluate drug and vaccine efficacy against SARS-CoV-2.

Plaat1l1 controls feeding induced NAPE biosynthesis and contributes to energy balance regulation in zebrafish.

This study identifies plaat1l1 as a key enzyme for feeding-induced NAPE biosynthesis in zebrafish, showing its role in energy balance regulation and obesity prevention.