R. Danev

Smart Parallel Automated Cryo Electron tomography

This paper develops an automated workflow using machine learning to perform in situ cryo electron tomography without human intervention.

Pharmacological hallmarks of allostery at the M4 muscarinic receptor elucidated through structure and dynamics

The paper presents cryo-EM structures of the M4 mAChR with ligands and modulators, revealing structural and dynamic insights into allosteric modulation and receptor activation mechanisms.

Parallel cryo electron tomography on in situ lamellae

This paper introduces a method to perform parallel cryo electron tomography on lamella samples, increasing throughput and data collection efficiency.

Structures of the human cholecystokinin 1 (CCK1) receptor bound to Gs and Gq mimetic proteins: insight into mechanisms of G protein selectivity

The paper determines cryo-EM structures of the CCK1R bound to Gs and Gq proteins, revealing conformational changes that enable promiscuous G protein coupling.

Dynamics of GLP-1R peptide agonist engagement are correlated with kinetics of G protein activation

The paper investigates how different GLP-1R agonists affect G protein activation kinetics through structural and functional analyses.

Structural and Functional Diversity among Agonist-Bound States of the GLP-1 Receptor

This study investigates how peptide agonist conformational plasticity influences GLP-1R activation through structural and functional analysis.

Structure and dynamics of semaglutide and taspoglutide bound GLP-1R-Gs complexes

This study determines cryo-EM structures of GLP-1R-Gs complexes bound with semaglutide and taspoglutide, revealing similar peptide binding modes but distinct 3D motions compared to GLP-1.

Evolving cryo-EM structural approaches for GPCR drug discovery

This study validates stable GPCR:Gs complexes without Nb35 stabilizers and evaluates 200kV vs 300kV cryo-EM imaging for drug discovery.

Routine sub-2.5 A cryo-EM structure determination of B-family G protein-coupled receptors

This study systematically optimizes cryo-EM parameters to routinely determine class B1 GPCR structures at better than 2.5 Å resolution.

Differential GLP-1R binding and activation by peptide and non-peptide agonists

The paper compares the binding and activation of the GLP-1 receptor by peptide and non-peptide agonists, revealing structural and functional differences that inform drug design.

Spectral DQE of the Volta Phase Plate

The paper quantifies the spectral dependence of signal loss in a Volta Phase Plate at different voltages and film thicknesses, finding a linear loss at higher resolution but an unclear cause.

Fast and accurate defocus for improved tunability of cryo-EM experiments

This paper introduces a method to modulate defocus during cryo-EM movie acquisition, enabling per-frame defocus adjustments for improved experimental tunability.

Cryo-EM structure of haemoglobin at 3.2 A determined with the Volta phase plate

The paper determines the 3.2 Å structure of human haemoglobin using a Volta phase plate, demonstrating its applicability to smaller protein complexes than previously possible with conventional methods.

Using the Volta phase plate with defocus for cryo-EM single particle analysis

This paper extends the use of the Volta phase plate in cryo-EM by incorporating defocus to improve data acquisition and achieve high-resolution 3D reconstructions.