Modelling cancer immunomodulation using epithelial organoid cultures

Yotam E. Bar-Ephraim; Kai Kretzschmar; Priyanca Asra; Evelien de Jongh; Kim E. Boonekamp; Jarno Drost; Joost van Gorp; Apollo Pronk; Niels Smakman; Inez J. Gan; Zsolt Sebestyén; Jürgen Kuball; Robert G.J. Vries; Hans Clevers

doi:10.1101/377655

Cancer BiologybioRxivOct 4, 2018

Preprint

Modelling cancer immunomodulation using epithelial organoid cultures

Yotam E. Bar-Ephraim iD, Kai Kretzschmar iD, Priyanca Asra, Evelien de Jongh, Kim E. Boonekamp, Jarno Drost iD, Joost van Gorp, Apollo Pronk, Niels Smakman, Inez J. Gan, Zsolt Sebestyén iD, Jürgen Kuball iD, Robert G.J. Vries, Hans Clevers iD

Abstract

Here we utilize organoid technology to study immune-cancer interactions and assess immunomodulation by colorectal cancer (CRC). Transcriptional profiling and flow cytometry revealed that organoids maintain differential expression of immunomodulatory molecules present in primary tumours. Finally, we established a method to model antigen-specific epithelial cell killing and cancer immunomodulation in vitro using CRC organoids co-cultured with cytotoxic T cells (CTLs).

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