cryo-EM

This study determines the cryo-EM structures of human claudin-18.2 bound to therapeutic antibodies, revealing key residues involved in antibody recognition.

This study reports cryo-EM structures of the 50S ribosome with EngA-bound GTPγS or GDP, revealing conformational changes in EngA and a novel SLJ motif during ribosome assembly.

This study determines the atomic structure of mycobacteriophage SWU1 using cryo-EM, revealing key architectural features critical for virion assembly and infection.

The paper identifies bacterial MVP homologs and reports cryoEM structures of vaults from Corallococcus praedator and a Deltaproteobacteria bacterium, revealing novel symmetries.

The paper reports the cryo-EM structures of an Acinetobacter baumannii podovirus, revealing its capsid maturation mechanism and providing a reference for phage engineering.

This study investigates the stepwise assembly and mechanical stability of nsP3 helical scaffolds in Chikungunya Virus using HS-AFM and EM, revealing domain-specific contributions to scaffold formation and function.

The paper identifies detergent LMNG as stabilizing a weak FZD-G protein interface, revealing that FZDs inherently weakly couple to G proteins and preferentially signal through DVL.

This study investigates how tau protein regulates motor proteins on microtubules using single-molecule imaging, cryo-EM, and MD simulations, revealing distinct mechanisms for inhibiting kinesin-1 and dynein.

This study identifies how LRRC58 senses cysteine through structural changes that regulate CDO1 binding and cysteine homeostasis.

This study presents cryo-EM structures of RtcB2 bound to damaged E. coli 30S subunits, revealing how the enzyme recognizes and repairs ribosomal RNA.

This study determines cryo-EM structures of FcR bound to IgM and IgA, revealing its binding mechanism and evolutionary insights into pIg receptor recognition.

This study reports cryo-EM structures of human PAT-1 with inhibitors to elucidate their inhibition mechanisms and domain interactions.

The paper investigates how oligodendrocytes contribute to the formation of α-synuclein filaments in multiple system atrophy by comparing seeded aggregation in different cell types.

This study determines the cryo-EM structures of Cereibacter sphaeroides ferrochelatase, revealing oligomeric states that occlude the substrate-binding site.

The paper determines the near-atomic structures of spirochete flagellar filaments, revealing how sheath proteins generate curvature and enable functional specialization.

This study determines the structure of human GluD1 and elucidates its ligand-gating mechanism using cryoEM and electrophysiology.

This paper introduces cryoSHIELDs, tunable peptides that improve cryo-EM sample preparation by modulating the air-water interface, enhancing particle distribution and complex integrity across multiple protein systems.

The paper reports a cryo-EM structure of a nitrogenase-PII supercomplex in methanogens, revealing a metabolite-gated assembly mechanism for nitrogenase inhibition.

This study identifies AHA, a conserved archaeal ribosome-associated factor involved in hibernation and energy sensing, linking prokaryotic translational silencing to eukaryotic energy sensing.

The paper determines the Cryo-EM structure of the Arabidopsis thaliana V-type ATPase, revealing its catalytic cycle and organelle-specific interactions with TLDc proteins.

This study reveals the structure and bundling mechanism of SMF-1, a CUP pilus in Stenotrophomonas maltophilia, showing it forms antiparallel bundles that initiate biofilm formation.

The paper presents cryo-EM structures of mitochondrial transcription initiation complexes, showing how TFAM and PolRMT interact with upstream DNA to regulate transcription initiation and specificity.

The paper characterizes pseudaminic acid modifications on the lateral flagellar filament of Vibrio alginolyticus and shows Maf is required for filament formation.

The paper presents a method for high-resolution cryoEM of nucleosomes in mammalian cell nuclear extracts without requiring thin sectioning.

This study reports cryo-EM structures of hibernating ribosomes from four Gram-negative bacteria, revealing how hibernation factors inhibit translation.

This study determines the structural basis of SAMD9 autoinhibition and explains how pathogenic mutations disrupt this mechanism to cause disease.

This study identifies structural adaptability in HIV-2 Vif that enables broad antagonism of APOBEC3G through conformational selection.

The paper presents the cryo-EM structure of Legionella pneumophila's MCC and identifies substrate-induced filamentation, suggesting a role in bacterial life cycle differentiation.

This study determines the cryo-EM structure of HIV-1 capsid lattice with lenacapavir, showing how it disrupts capsid formation and function during virion maturation.

The paper presents cryo-EM structures of a Blm10:13S proteasome intermediate, revealing parallel assembly pathways for the proteasome core particle involving Blm10 and Pba1/Pba2.