flow cytometry

This study shows that platelet extracellular vesicles containing mitochondria are internalized by neutrophils, altering their activation, adhesion, migration, and phagocytic capacity.

The paper identifies CASP3-GSDME-mediated trophoblast pyroptosis as a driver of systemic inflammation in early-onset preeclampsia and shows Vitamin D suppresses this process.

A2-CAR Tregs reduce BDC2.5 T cell engraftment, proliferation, and cytokine production, protecting mice from diabetes and inducing systemic tolerance.

This study identifies intra-arterial CD14+ monocytes as novel early sources of IFN-γ in hyperacute stroke, challenging the traditional view that adaptive immune cells are the main contributors.

The paper examines inhibitory NK receptors on γδ T cells in Ugandan children, finding that some enhance and others reduce antimalarial responses to repeated infections.

The paper tests the assumption of constant fitness effects in microbial populations and finds frequency-dependent effects are common, with negative frequency dependence and non-transitive fitness outcomes.

The paper examines B cell dynamics in different meningeal compartments of a progressive MS mouse model, identifying anti-CD20 resistant dural B cells that persist in the brain during remission.

This study identifies resistance mechanisms to BH3-mimetics in DLBCL and shows that combining BCL2 inhibition with non-canonical NF-κB inhibition overcomes microenvironment-mediated resistance.

This study identifies and characterizes HSV1-specific CD8+ T cells in zebrafish, showing an effective primary and memory response to an H-2Kb restricted epitope.

This study identifies an 82-gene transcriptomic signature predicting immune checkpoint inhibitor resistance in metastatic cutaneous melanoma using tumor and immune cell profiling.

This study identifies a pro-inflammatory role of Granzyme K-producing bystander CD8+ T cells in AML, which promote IL-8 secretion and may exacerbate disease progression.

This study shows that CD24a knockout in murine triple-negative breast cancer cells transforms the tumor microenvironment from cold to hot by increasing immune cell infiltration and reducing suppressive cells.

This paper introduces SpheroMap Cytometry, a spatial flow cytometry method to analyze immune cell infiltration in PDAC spheroids, revealing hypoxia-driven changes in T-cell distribution and function.

This study introduces SpheroMap Cytometry to analyze spatial CAF subsets in PDAC spheroids, revealing hypoxia's role in myCAF differentiation and stromal architecture.

The paper identifies profibrotic monocyte-derived alveolar macrophages as a biomarker and therapeutic target in SSc-ILD by analyzing BAL fluid and lung explants.

This study shows that Staphylococcus aureus infection increases NKG2A+ NK cells with higher IFN-γ production in humans, potentially driving inflammation and disease pathogenesis.

The paper identifies CD8 T cells in HIV-positive individuals that promote endothelial inflammation via granzyme-dependent PAR1 activation, linking immune activation to cardiovascular risk.

This study identifies CCR2+ neutrophils in atherosclerotic plaques that promote instability through ROS production and NET release, and shows that targeting CCR2 reduces plaque destabilization.

The paper identifies an IFN-STAT1-CYBB axis that mediates protective crosstalk between plasmacytoid dendritic cells and neutrophils during Aspergillus fumigatus infection.

The paper evaluates how the functional avidity of anti-B7H3 CAR-T constructs relates to their ability to trigger effector function against tumors with varying antigen density.

The paper re-evaluates the identity of CD4+ CD56+ CD16+ cells in human PBMC, finding they resemble NKT cells rather than exTreg, and explores Treg stability and Tr17 induction for therapeutic applications.

This study shows TRPV1+ sensory neurons protect against influenza by modulating myeloid cell responses and reducing immunopathology.

This study characterizes the immune subset composition of umbilical cord blood mononuclear cells and compares them to adult peripheral blood, revealing distinct immune profiles and developmental trends.

The paper shows that Mtb immunization protects against BCG infection despite inducing M-MDSC, and ATRA reduces M-MDSC to enhance immunity.

This study shows that nanofibrillar cellulose hydrogels preserve T cell function better than Matrigel or basement membrane extract in 3D cultures.

This study investigates how maternal opioid use disorder with and without hepatitis C infection affects placental structure, immune function, and cell-cell communication.

The paper examines T cell changes in mammary glands during pregnancy and lactation, finding an expansion of T effector cells and similarities to intestinal T cells.

This paper evaluates contrast agents for in vivo fluorescence detection of circulating tumor cells, finding that while they show high uptake, they also label non-cancer cells, reducing specificity.

The paper introduces an open-source R-based workflow to improve reproducibility and scalability of fluorescent reporter gene analysis in plant protoplasts using flow cytometry.

The paper shows Chlamydia activates B cells across species to become APCs that elicit strong T cell immunity against pathogens and tumors.