fluorescence / live-cell microscopy

This study demonstrates MINFLUX fluorescence nanoscopy's ability to achieve sub-10 nm resolution for protein localization in thick mouse brain tissue sections.

This study uses 3D super-resolution microscopy to analyze PSD95 nanoarchitecture in the mouse brain, revealing diffuse supercomplexes outside synapses and distinct subtypes within synapses.

This study shows that septins regulate Rga4 localization during the cell cycle in fission yeast, controlling Cdc42 activation dynamics and bipolar growth.

This paper develops a genetically encoded fluorescent biosensor, Malibu, to measure intracellular malonyl-CoA dynamics in single cells, showing increased responses to fatty acid biosynthesis inhibitors.

This study develops a fluorescent BMPP probe to detect triglyceride metabolism in white blood cells ex vivo, showing dose-dependent uptake and export.

This study uses plasmon-enhanced fluorescence to analyze the kinetics and dynamics of single-molecule multivalent interactions, revealing how spacer length and flexibility affect binding strength and dynamics.

This study uses single-molecule fluorescence imaging to investigate how eIF4F recognizes and activates mRNA caps, revealing allosteric communication and subunit functions in the process.

This study compares super-resolution microscopy techniques for imaging tightly packed microcolonies of an obligate intracellular bacterium, finding 3D-STED best for axial resolution and differences in bacterial shape across cell lines.

This paper predicts single-cell phenotypes from nuclear morphology using pre-labeled microscopy data, achieving high accuracy for some phenotypes but facing generalizability challenges.

SubCell is a deep learning model for fluorescence microscopy that captures cellular morphology and protein organization, outperforming existing methods and enabling a multimodal approach to gene function.

The paper develops a whole-cell assay to measure kinact/KI values for PBPs in Streptococcus pneumoniae, showing results consistent with in vitro data and supporting its validity as an in vivo method.

The paper tracks real-time transcription-translation coupling in bacteria using single-molecule fluorescence microscopy and shows ribosome-RNAP interactions and their functional implications.

The paper describes a modified multi-color TIRF microscope that enables simultaneous IRM and single molecule TIRF imaging of microtubules and EB1 proteins.

This study uses PIE-FCCS to show that PIP2 enhances EGFR and EphA2 multimerization in live cells, revealing a role for anionic lipids in RTK interactions.

The paper develops and validates fluorescence-based CRISPRi tools for gene repression and live single-cell imaging in mycobacteria.

This paper evaluates genetically-encoded reporters in Yarrowia lipolytica, finding that mScarlet-I3 and Nanoluc luciferase provide the highest signal/noise ratios for gene expression studies.

This study uses fluorescence lifetime imaging to quantify TZM-HER2 binding and distribution in tumors with varying microenvironments, showing differences in drug delivery efficacy between cancer types.

This study improves agreement between smFRET experiments and molecular dynamics simulations by accounting for time-averaging, revealing hidden conformational states in proteins.

SPACe is an open-source tool for analyzing Cell Painting data, offering faster processing and better handling of cell-to-cell variation compared to existing software.

The paper investigates centromere reorganization in Drosophila species, finding rapid turnover of retroelements and satellite DNA over short evolutionary timescales.

This study identifies kinetochore-independent forces, particularly plus-end directed forces from pole-to-pole microtubules, as critical for acentric chromosome congression and alignment during mitosis in Drosophila.

The paper quantifies variability in manual and automated cell segmentation and tracking methods, showing their impact on phenotypic metrics and reproducibility.

This paper introduces a labeling method for long-term single-molecule tracking in living cells and demonstrates its application to study EGFR dynamics.

This paper introduces an immunofluorescence-based method for high-precision cell cycle identification at the single-cell level, offering an alternative to flow cytometry for analyzing cell cycle stages.

ABHD5 binds PNPLA3 more strongly than ATGL regardless of the I148M variant; steatosis from PNPLA3(148M) needs ATGL and is reversed by ABHD5 overexpression.

The paper introduces PRINCESS, a novel biosensor that simultaneously detects membrane contact sites and measures associated Ca2+ dynamics using splitFAST technology.

This study uses cryo-EM and smFRET to reveal structural and kinetic details of yeast TFIIIC engagement with tRNA promoter DNA motifs.

This study uses 3D nnUnet and Light Sheet Microscopy to automate cell tracking and quantify myocardial deformation in zebrafish over time.

The paper uses single-molecule imaging and molecular dynamics simulations to study early MET receptor activation in situ, revealing structural dynamics and a refined dimer model.

This paper describes a protocol for analyzing muscle fiber type and cross-sectional area in cryosections of whole lower mouse hindlimbs using immunofluorescence and histochemistry.