Ion channel regulation and function

This study reports the cryoEM structure of an open KATP channel with PIP2 bound, revealing two PIP2 binding sites and their role in channel regulation and neonatal diabetes.

This study investigates whether CALHM2 and CALHM4 form heteromers and characterizes their structural properties.

The paper investigates how spatial variations in sodium channel kinetics along the axon initial segment affect backpropagation of action potentials during orthodromic and antidromic stimulation.

The paper studies two mutations in the zebrafish Kcng4b gene, showing how they affect Kv channel function and lead to distinct developmental defects in ear development.

This paper generates a Kcnb1R306C mouse model to study the effects of a voltage-sensing mutation in KCNB1, finding altered neurological and neurobehavioral phenotypes.

The paper investigates how disease-causing gain-of-function mutations in the Slack potassium channel affect excitability differently in excitatory and inhibitory neurons.

This study investigates how PUFAs stabilize the selectivity filter of IKs channels, increasing their conductance and potentially treating Long QT Syndrome.

The paper investigates how the scorpion toxin AaH-II affects action potential generation in the axon initial segment of mouse neurons using high-resolution imaging techniques.

The paper shows that yeast Bxi1p/Ybh3p is a calcium channel in E. coli, and that gadolinium inhibits it, supporting its classification as a TMBIM family member.

This study demonstrates cooperative cAMP binding to resting HCN2 channels in native membranes using single-molecule fluorescence techniques, showing cooperativity precedes channel activation.

This paper introduces selective NaV1.6 and dual NaV1.6/NaV1.2 inhibitors that suppress excitatory neuronal activity ex vivo, showing selectivity over NaV1.1 and efficacy in seizure models.

This study investigates the interaction between N-type Ca2+ channels and BK channels in mouse neocortical layer-5 pyramidal neurons, revealing their functional coupling and impact on AP shape and Ca2+ influx.

The paper investigates how upregulated GIRK2 affects ethanol-induced changes in human neurons, showing it counteracts excitability and respiration changes.

This study identifies compound 6 as a potent Slack potassium channel activator effective in treating histamine-independent and chronic itch without motor side effects.

This study determines the structural basis of neuropeptide binding and gating in a DEG/ENaC channel using cryo-EM and mutagenesis.

The paper identifies the cellular and subcellular localization of Kv2.1 and Kv2.2 channels in DRG neurons, showing similarities between mice and humans.

This paper identifies critical molecular interactions for Nav1.2 channel fast inactivation by studying pathogenic SCN2A mutations and their biophysical effects.

This paper develops GeeC, a genetically encoded actuator that boosts L-type CaV1.2/1.3 channel function in cardiomyocytes and neurons, enhancing calcium influx and excitation-transcription coupling.

The paper measures human sodium current voltage-dependence at physiological temperature using patch-clamp experiments combined with a mathematical model, revealing a depolarized activation compared to previous studies.

The paper uses two Kv2 inhibitors to distinguish Kv2/KvS heteromer conductances from Kv2-only channels in neurons.

This paper uses AlphaFold to predict discrete conformational states of hERG channels, revealing structural features and drug interactions that improve drug safety screening.

This study uses computational modeling to show that hyperpolarization-activated inward currents (h-currents) can facilitate postinhibitory excitation in human motoneurons.

This study identifies LMAN2 as a regulatory protein that modulates redox-sensitive gating of Kv1.2 channels by altering activation kinetics and redox sensitivity.

This study examines the electrophysiological properties of human iPSC-derived vascular smooth muscle cells and identifies cell-autonomous effects of Cantu Syndrome mutations on KATP channel function and vascular compliance.

The paper investigates motifs in CaV2.1 subunits that regulate presynaptic abundance and preference, finding distinct motifs control abundance versus preference.

This study investigates whether ethanol's interaction with BK channel α subunit residue K361 mediates behavioral responses to alcohol in mice, finding no significant effects on ethanol-related behaviors or consumption.

This study uses voltage-clamp fluorometry to map conformational changes in the 5-HT3 serotonin-gated channel, linking them to electrophysiological states and agonist effects.

The paper investigates how asymmetric conformational changes in CaV1.3 channels mediate voltage- and calcium-dependent inactivation through distinct molecular mechanisms.

The paper determines the structure of Arabidopsis thaliana OSCA2.3 using cryo-EM, revealing conformational heterogeneity and a conserved interaction between the TM6a-TM7 linker and the Beam-Like Domain.

This study identifies a conserved interface in KATP channels critical for Mg-ADP activation through mutagenesis experiments and structural analysis.