Fungal and yeast genetics research

This paper develops a biosensor to visualize and manipulate PKA activity in fission yeast, revealing its dynamics during cell cycle phases.

This study demonstrates the use of alkaline-pH regulatable promoters for methanol-free expression of phytase in Komagataella phaffii, achieving higher production than the traditional AOX1 promoter.

The paper identifies new alleles and regulatory mechanisms of the fMAPK pathway in yeast through genetic screening and whole-genome sequencing.

This study shows Cbf11 and Mga2 work together to regulate lipid metabolism and mitotic fidelity in fission yeast, with similar single and double deletion phenotypes.

This study investigates how budding yeast adapts to sucrose transitions by analyzing cAMP signaling dynamics and their impact on growth and metabolism.

This paper compares the temporal dynamics of morphogenesis and gene expression during C. albicans filamentation in vitro and in vivo, identifying key differences in metabolic gene expression and hyphae-to-yeast transition rates.

This paper investigates the role of H3K4 methylation in Mucorales fungi, showing it regulates development, DNA repair, and virulence through genetic and phenotypic analyses of set1 knockout strains.

The paper expands a yeast cloning toolkit to include optimized signal peptides and surface display anchors for improved protein secretion and display in S. cerevisiae.

The paper genetically modifies Candida maltosa to study Efg1 function, finding it represses filamentation despite gene loss in the species.

The paper identifies Ppg1 as a phosphatase that regulates Far complex assembly to maintain gluconeogenic outputs during glucose depletion in yeast.

The paper analyzes PKA signaling in yeast using phosphoproteomics to show how different cAMP concentrations and conditions affect phosphorylation thresholds and growth states.

This paper develops a yeast artificial chromosome system in K. marxianus to enhance heterologous protein expression by incorporating disulfide bond formation modules, achieving record-high glucoamylase yields.

The paper presents a telomere-to-telomere genome assembly of S. japonicus, revealing a previously unknown second centromere-like region and revising the genome size estimate.

The paper shows that the fungal gene ENB1, acquired from a bacterial operon, enables iron uptake in W/S clade yeasts and evolved through horizontal transfer and gene loss.

The paper characterizes TLH gene families in four Fusarium oxysporum strains, identifying conserved motifs, phylogenetic clustering, and subtelomeric regions associated with genome stability.

This study investigates cold adaptation routes in Saccharomyces species by analyzing gene expression and allelic variation across multiple strains.

The paper shows Elm1 and Gin4 kinases regulate each other through direct binding and phosphorylation to control septin hourglass assembly and remodeling during different cell cycle stages in yeast.

The paper identifies 829 xenologs in 44 early diverging fungi, revealing patterns of horizontal gene transfer and its impact on fungal metabolism, adaptation, and immunity.

This study shows that the function of the C. albicans transcriptional repressor NRG1 varies by strain, affecting filamentation and virulence in different contexts.

The paper analyzes meiotic recombination landscapes across five yeast species, finding species-specific differences in recombination rates, crossover interference, and hotspot conservation.

The paper identifies a novel gene family, Snowball (snb), essential for fruiting body development in Agaricomycetes fungi, showing its role in differentiation through mutant analysis and transcriptomics.

The paper develops a bacterial toxin-antitoxin system to manipulate and visualize tandem gene repeat copy numbers in yeast.

This study investigates how hybridisation in Saccharomyces species affects antifungal drug resistance by identifying QTLs and potential causal genes involved in resistance to fluconazole, micafungin, and flucytosine.

This study investigates the role of the osaA gene in Aspergillus fumigatus, showing its impact on growth, conidiation, cell wall integrity, secondary metabolism, and virulence.

This paper develops and validates a CRISPR activation system in Nakaseomyces glabrata to study gene overexpression in pathogenesis and drug resistance.

This study profiles the effects of SAM1 and SAM2 gene deletions on S. cerevisiae by analyzing growth and gene expression changes across multiple conditions.

The paper improves the Z3EV promoter system to create the strongest yeast promoter, P36, which is 1.4 times stronger than TDH3 and can express up to 50% of total protein as a heterologous protein.

This paper expands the YESS system by creating integrative plasmids for modular and uniform activity reporting of PTM-enzymes in yeast.

This paper introduces CReATiNG, a method to build synthetic chromosomes from natural DNA in yeast, enabling chromosome recombination, modification, and deletion of non-adjacent regions.

The paper identifies recombination hotspots in Neurospora crassa controlled by idiomorphic sequences and meiotic silencing, showing dominant suppression is due to silencing of the active allele.