Fungal and yeast genetics research

This paper improves therapeutic protein secretion in Saccharomyces boulardii using genetic modifications and proteomics, achieving >10-fold higher secretion of a neutralizing peptide.

The paper investigates how PKA signaling coordinates growth and metabolism in yeast during anaerobic xylose fermentation by analyzing transcriptomic, lipidomic, and phosphoproteomic data.

The paper develops an open-source yeast chassis, OPENPichia, for synthetic biology by reverse engineering and characterizing industrial Pichia strains.

The paper characterizes the temperature responsiveness of hsp promoters in Neurospora crassa, identifying hsp30 as a highly tunable promoter for synthetic biology applications.

The paper shows that ploidy reduction in yeast populations evolved for maltose utilization is driven by an interaction between cell type and metabolism, with haploid-like transcriptional programs conferring fitness advantages.

The paper compares proteomes of three Saccharomyces cerevisiae strains grown on sucrose and glucose, finding Mnp1 is consistently higher on sucrose and opposite biological function patterns between strains.

This study uses CRISPR/Cas9 RNP to knockout the CcPka1 gene in Coprinopsis cinerea, revealing its role in nutrient signaling and fruiting body formation.

This paper designs and synthesizes a functional neochromosome in yeast that replaces a native chromosome arm, demonstrating genome plasticity and synthetic chromosome viability.

This paper shows that reducing Cdc14 phosphatase activity in Candida albicans impairs cell wall integrity, septation, hyphal differentiation, and virulence, making it a potential antifungal drug target.

This study identifies MoPtc1 and MoPtc2 as type 2C phosphatases that synergistically regulate MAPK pathways in Magnaporthe oryzae during pathogen-host interactions.

This study shows that constitutive activation of TORC1 signaling in Fusarium oxysporum reduces virulence by impairing invasive hyphal growth and pathogenicity in both plant and animal hosts.

The paper debugs a synthetic yeast chromosome and uses laboratory evolution to identify genomic rearrangements that suppress growth defects caused by synthetic modifications.

The paper constructs a synthetic yeast chromosome XI and demonstrates how non-coding DNA modifications affect cell fitness, enabling on-demand extrachromosomal circular DNA formation.

The paper shows that Saccharomyces cerevisiae maintains excess glycolytic enzyme capacity under glucose-limited conditions as an adaptation to environmental nutrient fluctuations.

The paper measures mutation rates in S. pombe using canavanine resistance and finds that spontaneous mutants predominantly have an R175C mutation in the any1 gene, which confers resistance through altered arginine transport and cell morphology.

The paper identifies that most MHD-only fungal TFs lack a DNA binding domain due to annotation errors, redefining the CeGAL family as a new TF family with Zn2C6-MHD domains.

The paper examines transcriptome responses to MMS-induced DNA damage in four Saccharomyces species, revealing species-specific gene expression changes and functional genomic insights.

This study clones and expresses a 9-lipoxygenase gene from Solanum tuberosum in Pichia pastoris to produce recombinant protein for biodegradable fungicide development.

The paper investigates how alternative transcription start sites regulate the production of cytosolic and mitochondrial branched-chain aminotransferase proteoforms in Kluyveromyces marxianus, showing condition-specific regulation by transcription factors.

The paper presents full-length telomere-to-telomere genome assemblies for two Aspergillus fumigatus strains using long-read sequencing technologies.

This study identifies a dominant any1 mutation that confers strong canavanine resistance in fission yeast, enabling its use as a genetic selection marker.

The paper identifies two genes regulating temperature-dependent morphogenesis in Z. tritici, showing that intracellular osmotic stress drives these transitions.

The paper shows that yeast cells lacking Met15 can assimilate inorganic sulfur via Yll058w, but require exogenous methionine or hydrogen sulfide chelators to grow, challenging the traditional view of MET15 as essential for sulfur assimilation.

The paper identifies Spok1, a fungal spore killer protein, as a DNA-targeting agent that causes killing in fungi and functions across bacteria and yeast.

The paper shows that meiosis in Aspergillus fumigatus has the highest known crossover rate, producing ~29 crossovers per chromosome, and can generate azole-resistant haplotypes.

This study combines pan-, population-, and phylo-genomic analyses of Aspergillus fumigatus to reveal population structure, lineage-specific diversity, and ecological drivers of virulence.

The paper identifies KTD1 as a novel defense factor in Saccharomyces cerevisiae that confers resistance to the K28 killer toxin.

The paper investigates how wtf4 meiotic drivers in S. pombe use dual transcriptional regulation and selective protein exclusion to ensure their transmission through gametes.

This study successfully humanizes the entire glycolytic pathway in yeast, revealing conserved functions and context-dependent responses.

The paper investigates stochastic switching between two heritable cell states in Candida albicans using microfluidics and fluorescent reporters, identifying two stochastic steps involving Wor1 autoregulation.