Advanced Fluorescence Microscopy Techniques

This paper demonstrates a method to achieve high-resolution imaging over a large field of view using SRRF and Mesolens, achieving 446.3 nm resolution with minimal error.

This study explores mScarlet-based red fluorescent proteins for developing a calcium indicator with fluorescence lifetime contrast, but finds that lifetime contrast is lost in mammalian cells compared to bacterial lysates.

This study demonstrates MINFLUX fluorescence nanoscopy's ability to achieve sub-10 nm resolution for protein localization in thick mouse brain tissue sections.

This paper develops a genetically encoded fluorescent biosensor, Malibu, to measure intracellular malonyl-CoA dynamics in single cells, showing increased responses to fatty acid biosynthesis inhibitors.

This paper develops a high-resolution microscopy method for imaging live biological samples at sub-zero temperatures to study cold-adapted species under physiological conditions.

This paper introduces a method for highly multiplexed fluorescence microscopy using tunable semiconducting polymer dots, enabling imaging of up to 21 targets in a single round.

This study compares super-resolution microscopy techniques for imaging tightly packed microcolonies of an obligate intracellular bacterium, finding 3D-STED best for axial resolution and differences in bacterial shape across cell lines.

The paper develops an all-optical system to measure calcium transport properties in dendrites, finding a mean length constant of ~5.8 m and a half-life of ~1.7 s.

PISA enables 3D single molecule light sheet imaging of coverslip-mounted samples with high-throughput capabilities and improved sensitivity for biochemical assays.

The paper describes a modified multi-color TIRF microscope that enables simultaneous IRM and single molecule TIRF imaging of microtubules and EB1 proteins.

This paper develops a hydrogel-based biosensor for continuous nicotine measurement in human biofluids, achieving detection at sub-100 nM levels with high stability and sensitivity.

The paper shows that high innervation density of cross-reacting transmitters causes non-specific activation of GPCR-based sensors, requiring specific innervation silencing for accurate interpretation.

This paper introduces a microwave-assisted expansion microscopy protocol that significantly reduces processing time while maintaining resolution and expansion efficiency across different organisms.

The paper develops a super-resolution microscopy technique to image neuronal morphology in behaving animals, revealing fast spinule dynamics and PSD-95 cluster correlations.

This paper introduces a labeling method for long-term single-molecule tracking in living cells and demonstrates its application to study EGFR dynamics.

The paper introduces a novel rhodopsin-based voltage indicator, 2Photron, enabling simultaneous two-photon recording of spiking and subthreshold voltage with GCaMP in awake mice.

This study develops a method to image the spinal cord in awake, behaving mice over long periods, enabling monitoring of neural activity and behavior.

This paper enables long-term super-resolution imaging of ER dynamics in living cells using low irradiation and neural network-based image restoration.

This study introduces 4x and 12x 3D-ExM methods for super-resolution microscopy, achieving nanometer-scale resolution for visualizing cellular structures.

This paper introduces a single objective light-sheet microscope for high-resolution in vivo brain imaging in Drosophila, achieving improved resolution and reduced photobleaching and phototoxicity.

This paper introduces long axial-range double-helix PSFs for 3D super-resolution imaging, enabling stitching-free imaging of whole mammalian cells with improved speed and resolution.

The paper introduces FLIPR, a remote focusing technique that improves light efficiency and enables high-speed volumetric voltage imaging at 500 volumes per second.

This study evaluates subcellular targeting of ArcLight, a GEVI, using different targeting sequences to enhance signal localization without affecting voltage sensitivity.

The paper uses automated live-cell single-molecule tracking to study transcription factor dynamics in mouse enteroid monolayers, revealing contrasting diffusive behaviors that correlate with differentiation stages.

This paper introduces SPARCLS, a high-speed 3D random-access scanning method achieving 340 kHz point rate, and demonstrates its application in imaging synaptic events in mammalian and zebrafish models.

This study quantifies hemodynamic occlusion effects in two-photon imaging and finds they can rival activity sensor signals and show spatial and behavioral variability.

GESIAP3.0 enables precise quantitative analysis of neuromodulatory synaptic transmission in behaving animals by correcting for tissue movement and optimizing synaptic information extraction.

This study reproduces a technique using absorbing molecules to achieve in vivo tissue transparency in live mice, identifies key experimental factors for reproducibility, and observes a new transparency effect in the back.

The paper introduces Shielded DNA-PAINT, a method to reduce non-specific DNA-PAINT probe binding in cells, achieving a 5-fold reduction in non-specific events in the nucleus.

This study uses single-molecule tracking to screen FDA-approved drugs for effects on EGFR mobility and clustering, identifying both known and novel compounds that impact EGFR function.