Advanced Fluorescence Microscopy Techniques

The paper uses molecular dynamics simulations to analyze hydrogen bond dynamics in a maltose biosensor, identifying key factors distinguishing its bright and dark states.

This paper introduces MIET-PAINT, a method combining DNA-PAINT and Metal-Induced Energy Transfer to achieve nanometer-scale 3D imaging with high multiplexing capability.

The paper describes an open-source miniature 2-photon microscope for recording calcium dynamics in freely behaving mice during various experimental conditions.

The paper introduces an improved open-source light-sheet microscope, mesoSPIM, with enhanced resolution, throughput, and compatibility with cleared samples.

This study develops a highly sensitive GEVI called rEstus to measure electrical activity in non-excitable cells and demonstrates its use in detecting voltage fluctuations and gap-junction coupling.

The paper develops ultrasensitive cAMP and Ca2+ indicators to measure their dynamics in neurons and identifies their roles in signaling and behavior.

The paper develops a non-invasive NIR imaging technique to visualize live plant cell nuclei using phytochrome-derived autofluorescence, applicable across multiple plant species.

The paper describes an open-source fluorescent imaging system (openIVIS) that can be used for in vivo and in vitro applications, demonstrating its versatility and low cost.

This study develops a fluorescent opioid biosensor to measure fentanyl levels in mice and links pharmacokinetics to behavioral patterns using computational ethology.

The paper establishes dual-color sptPALM for tracking two plasma membrane proteins in Arabidopsis thaliana cells using optimized fluorescent proteins.

This paper introduces a beam-based focus stabilisation method using astigmatism, compatible with various objective lenses and offering nanometre precision and extended operating range.

The paper introduces MINSTED localization, a method for tracking labeled biomolecules with nanometer and millisecond precision using STED microscopy.

The paper derives a depth-dependent axial scaling theory for light microscopy under refractive index mismatch and provides software to correct axial deformation in imaging data.

This paper develops an optogenetic method to release single molecules in live cells, enabling controlled delivery of functional proteins and reconstitution of cellular functions at the single molecule level.

This study compares the photostability of StayGold with three other FPs under different illumination modes in microscopy, finding that StayGold maintains superior photostability in multi-beam LSCM but shows variable performance in single-beam LSCM.

This paper introduces a 4Pi MINFLUX technique that achieves high spatio-temporal localization precision for single fluorophores, revealing nanoscale diffusion heterogeneities in solutions.

This paper develops O2-independent bioreporters using KOFP-7, demonstrating fluorescence in anaerobic conditions for bacteria like V. diazotrophicus.

This paper introduces SUM-PAINT, a high-throughput imaging method for single-protein spatial resolution, and discovers a new synapse type through extensive multiprotein analysis.

This paper presents an oblique plane microscope compatible with a commercial base, showing minimal impact on resolution and data fidelity despite objective lens displacement.

The paper introduces designed fluorescent protein cages that target specific subcellular locations in mammalian cells for imaging, demonstrating their ability to penetrate cells and be detected with confocal microscopy.

This study compares three monomeric variants of StayGold to evaluate their photostability, brightness, and dispersibility, highlighting tradeoffs in fluorescent protein technology.

FiPhA is an open-source platform for fiber photometry analysis that simplifies data processing, visualization, and quality control for behavioral neuroscience research.

This study demonstrates that fluorescence lifetime can accurately measure neuromodulator dynamics across animals and over time, overcoming limitations of intensity-based measurements.

This paper introduces a method to map amino acid positions and post-translational modifications in single protein molecules with sub-nanometer resolution using FRET-based single-molecule protein fingerprinting.

FluoroTensor uses deep learning to identify and track colocalized molecules and their stoichiometries in multi-color single molecule imaging.

The paper introduces a novel genetically-encoded sensor for PAC1 receptor activation, demonstrating its utility in vitro and in vivo across species.

This paper introduces a new fluorescent sensor, oROS-HT, for real-time H2O2 monitoring with subcellular resolution and multi-parametric imaging capabilities.

The paper develops a Cre-dependent reporter mouse, FL-AK, for quantitative imaging of PKA activity dynamics in vivo and ex vivo.

The paper introduces Solaris, a hybrid voltage indicator with improved sensitivity for imaging membrane potential in cultured neurons.