C. MacDonald

High resolution live cell imaging to define ultrastructural and dynamic features of the halotolerant yeast Debaryomyces hansenii

This study uses high-resolution live cell imaging to characterize sub-cellular structures and dynamic processes in the halotolerant yeast Debaryomyces hansenii, providing a template for studying genetically recalcitrant organisms.

iPAR: a new reporter for eukaryotic cytoplasmic protein aggregation

The paper introduces iPAR, a fluorescent reporter for studying cytoplasmic protein aggregation in yeast, showing aggregates are mobile and not inherited by daughter cells.

The full spectrum of OCT1 (SLC22A1) mutations bridges transporter biophysics to drug pharmacogenomics

This study examines OCT1 mutations to understand their impact on drug response and identifies population-specific allele frequencies linked to LDL cholesterol levels.

The phosphatase Glc7 controls eisosomal response to starvation via posttranslational modification of Pil1

The paper shows Glc7 dephosphorylates Pil1 in response to glucose starvation, modulating eisosome function and nutrient transporter retention.

Correlating viscosity and molecular crowding with fluorescent nanobeads and molecular probes: in vitro and in vivo

The paper investigates intracellular viscosity and molecular crowding using fluorescent nanobeads and molecular probes in vitro and in live yeast cells.

Determining the optimal SARS-CoV-2 mRNA vaccine dosing interval for maximum immunogenicity

This study finds that longer SARS-CoV-2 vaccine dosing intervals (≥30 days) are associated with higher antibody concentrations and viral neutralization at 6 months post-first dose.

The Rpd3-complex regulates expression of multiple cell surface recycling factors in yeast

The paper identifies Rpd3-complex subunits that regulate recycling of surface proteins in yeast by modulating target gene expression.

Investigating molecular crowding during cell division in budding yeast with FRET

This study quantifies molecular crowding during budding yeast cell division using FRET sensors and microscopy, revealing crowding hotspots in daughter cells and potential mechanisms involving inherited organelles.

Recycling of cell surface membrane proteins from yeast endosomes is regulated by ubiquitinated Ist1

The paper identifies a conserved mechanism for endosomal recycling of nutrient transporters in yeast, showing that ubiquitinated Ist1 and associated factors regulate this process.

Fur4 mediated uracil-scavenging to screen for surface protein regulators

The paper develops a yeast-based assay to screen for surface protein regulators by measuring uracil-scavenging activity and identifies factors involved in membrane trafficking and transcriptional control.

Endosomal cargo recycling mediated by Gpa1 and Phosphatidylinositol-3-Kinase is inhibited by glucose starvation

The paper shows glucose starvation inhibits endosomal cargo recycling by altering PI3K activity through Gpa1 and Gpa2 interactions.

Shark conservation risks associated with the use of shark liver oil in SARS-CoV-2 vaccine development

This paper examines the conservation risks of using shark liver oil-derived squalene in SARS-CoV-2 vaccines and suggests sustainable alternatives.

Molecular crowding in single eukaryotic cells: using cell environment biosensing and single-molecule optical microscopy to probe dependence on extracellular ionic strength, local glucose conditions, and sensor copy number

This study uses FRET biosensing and microscopy to measure molecular crowding in yeast cells under varying glucose and ionic conditions, revealing uniform cytosolic crowding and sensor copy number dependencies.

A glucose starvation response governs endocytic trafficking and eisosomal retention of surface cargoes

The paper shows glucose starvation in yeast increases internalization of surface cargoes while suppressing their recycling, and upregulates eisosomal factors to aid nutrient uptake recovery.

The chemistry and pharmacology of putative synthetic cannabinoid receptor agonist (SCRA) new psychoactive substances (NPS) 5F-PY-PICA, 5F-PY-PINACA, and their analogues

This study synthesizes and characterizes new psychoactive substances (NPS) and evaluates their pharmacology at cannabinoid receptors.

COPI Mediates Recycling Of An Exocytic SNARE From Endosomes By Recognition Of A Ubiquitin Sorting Signal

This study shows COPI mediates recycling of the SNARE Snc1 from early endosomes to the Golgi via recognition of a polyubiquitin sorting signal.