J. Frederick

Formamide denaturation of double-stranded DNA for fluorescence in situ hybridization (FISH) distorts nanoscale chromatin structure

This study shows that formamide in 3D FISH distorts nanoscale chromatin structure, while alternative methods like RASER-FISH and CRISPR-Sirius have minimal impact.

Microgroove substrates unveil topography-driven, dynamic 3D nuclear deformations

This study uses microgroove substrates to show that adherent cells exhibit dynamic 3D nuclear deformations driven by adhesion, with implications for diagnosing nuclear mechanical disorders.

Tunable biomimetic materials elaborated by ice templating and self-assembly of collagen for tubular tissue engineering

This study develops tunable collagen-based tubular materials using ice templating and self-assembly for tissue engineering, showing successful colonization by human cells.

Multiple mechanisms contribute to isolation-by-environment in the redheaded pine sawfly, Neodiprion lecontei

This study identifies multiple mechanisms contributing to isolation-by-environment in the redheaded pine sawfly by analyzing host-associated differentiation and ecological barriers.

Straining the root on and off triggers local calcium signaling

This study applies lateral pressure to plant roots to investigate calcium signaling, finding that straining triggers localized calcium signals that attenuate with repeated stimulation.

Functional mRNA delivery to hematopoietic stem and progenitor cells in vivo.

This study demonstrates in vivo delivery of functional mRNA to hematopoietic stem cells (HSC) using lipid nanoparticles, achieving transfection in ~25% of mouse HSC with potential for therapeutic applications.