S. Chang

A bipartite interaction with the processivity clamp potentiates Pol IV-mediated TLS

This study shows that a unique secondary contact of Pol IV with the processivity clamp enhances its ability to perform translesion synthesis past strong replication blocks.

Negatively charged α-Synuclein condensate modulates partitioning of molecules

This study shows that negatively charged α-Synuclein condensates influence molecular partitioning based on charge differences, revealing electrostatic effects on phase separation.

The Origins of ATP Dependence in Biological Nitrogen Fixation

This study experimentally reconstructs an ancestral nitrogenase enzyme and shows it strictly requires ATP hydrolysis for N2 reduction, decoupling cofactor selection from environmental availability.

Architecture of intact mammalian pyruvate dehydrogenase complex revealed by in-situ structural analysis

This study reveals the in-situ architecture of the mammalian pyruvate dehydrogenase complex using cryo-electron tomography, showing dynamic interactions and variable peripheral compositions.

Tumor-selective effects of active RAS inhibition in pancreatic ductal adenocarcinoma

This study evaluates the therapeutic potential of RMC-7977, a broad-spectrum RAS inhibitor, in pancreatic ductal adenocarcinoma models, showing strong anti-tumor effects with minimal impact on normal tissues.

De novo design of highly selective miniprotein inhibitors of integrins αvβ6 and αvβ8

This paper designs highly selective miniprotein inhibitors for integrins αvβ6 and αvβ8 with picomolar affinity and therapeutic potential in a mouse fibrosis model.

Myostatin gene deletion alters gut microbiota stimulating fast-twitch glycolytic muscle growth

This study shows that Myostatin gene deletion in pigs alters gut microbiota, increasing SCFAs which stimulate fast-twitch glycolytic muscle growth through the Akt/mTOR pathway.

Comprehensive Multi-omics Analysis Reveals the Core Role of Glycerophospholipid Metabolism in Rheumatoid Arthritis Development

This study identifies glycerophospholipid metabolism as a key factor in rheumatoid arthritis development through multi-omics analysis of gut microbiota, metabolites, and gene expression.

Impact of combinatorial histone modifications on acetyllysine recognition by the ATAD2 and ATAD2B bromodomains

This study investigates how ATAD2 and ATAD2B bromodomains recognize acetylated lysine on histones in the presence of other modifications, revealing distinct binding modes and mechanisms.

Bioinformatics analysis of immune-related prognostic genes and immunotherapy in renal clear cell carcinoma

The paper identifies 4 immunoprognostic genes in ccRCC using bioinformatics analysis of public databases and validates their association with prognosis and immunotherapy response.

Replication stalling activates SSB for recruitment of DNA damage tolerance factors

This study shows that replication stress causes SSB to recruit TLS polymerase Pol IV and other SIPs to stalled forks, suggesting a general mechanism for repair factor enrichment.

Fast volumetric imaging with line-scan confocal microscopy by an electro-tunable lens

This paper introduces an electro-tunable lens in line-scan confocal microscopy to enable fast volumetric imaging with high acquisition rates and depth resolution.

Roflumilast, a Phosphodiesterase-4 Inhibitor, Ameliorates Sleep Deprivation-Induced Cognitive Dysfunction in C57BL/6J Mice

This study shows that Roflumilast improves cognitive dysfunction caused by sleep deprivation in mice by modulating PDE4B and related signaling pathways.

Refractive-index matching enhanced polarization-sensitive optical coherence tomography quantification in human brain tissue

This study improves PS-OCT quantification in human brain tissue using refractive index matching, reducing noise and improving birefringence measurements in both white and gray matter.

Cryo-EM structure of the human TACAN channel in a closed state

The paper presents the cryo-EM structure of the human TACAN channel in a closed state and identifies key residues and cholesterol interactions involved in its function.

Dynamic assembly of the calcium hemostasis modulator 1 channel gates ATP permeation

This study reports cryo-EM structures of CALHM1 channels and proposes a mechanism for ATP permeation through dynamic N-helix movement.

Discovery of the first selective M4 muscarinic acetylcholine receptor antagonists with in vivo anti-parkinsonian and anti-dystonic efficacy

This paper discovers the first selective M4 muscarinic acetylcholine receptor antagonists and confirms their anti-parkinsonian and anti-dystonic efficacy in rodent models.

A critical role of stable grain filling rate in maximizing rice yield revealed by whole plant carbon nitrogen interaction modeling

This study develops a whole-plant carbon nitrogen interaction model to show that a stable grain filling rate from flowering to harvest maximizes rice yield, validated in high-yielding cultivars.

Transcriptional Atlas of Ileal-Anal Pouch Immune Cells from Ulcerative Colitis Patients

The paper uses single-cell RNA-sequencing to analyze immune cells in ileal pouches and colons of UC patients, identifying specific cell types and their association with inflammation and treatment response.

Structural insight into outer membrane asymmetry maintenance of Gram-negative bacteria by the phospholipid transporter MlaFEDB

The paper determines cryo-EM structures of MlaFEDB and reveals its phospholipid transport mechanism, identifying functional residues and providing insights for antimicrobial drug development.

Chemoinformatic-guided engineering of polyketide synthases

This study uses chemoinformatics to engineer polyketide synthases for producing saturated β-carbon compounds, finding a correlation between chemical similarity and product titers.

Multiscale Characterization of Complex Binding Interactions of Cellulolytic Enzymes Highlights Limitations of Classical Approaches

This study uses single-molecule assays and simulations to show how CBM1 binding affects cellulase activity on different cellulose allomorphs, highlighting limitations of classical binding models.

Targeted Eicosanoid Profiling Reveals MicroRNA-155 Shifts PGE2/PGD2 Balance Towards Oncogenic State

This study shows miR-155 shifts PGE2/PGD2 balance towards an oncogenic state by regulating enzymes involved in prostaglandin synthesis.

Compartmentalization of the replication fork by single-stranded DNA binding protein regulates translesion synthesis

The paper shows that SSB remodeling at stalled replication forks enables Pol IV localization, which is essential for TLS on both strands by overcoming the gatekeeping role of the Pol III epsilon subunit.

Conventional dose rate spatially-fractionated radiation therapy (SFRT) treatment response and its association with dosimetric parameters - A preclinical study in a Fisher 344 rat model

This study identifies dosimetric parameters associated with tumor response and body weight change in spatially-fractionated radiation therapy using a rat model.

Characterization of an ECF56-family sigma factor from Streptomyces venezuelae reveals a highly conserved regulome

This study characterizes the ECF56-family sigma factor from Streptomyces venezuelae, showing its C-terminal extension is conserved and regulates a highly conserved regulome across Actinobacteria.

Effect of Perfluorooctanesulfonic acid (PFOS) on immune cell development and function in mice

This study examines the effects of PFOS exposure on immune cell development and function in mice, finding no significant suppression of immune responses or weight loss.

Developing a rapid and highly efficient cowpea regeneration and transformation system using embryonic axis explants

This study develops a rapid and efficient cowpea transformation system using embryonic axis explants, achieving transgenic shoot frequencies of 4.5 to 37%.

A gatekeeping function of the replicative polymerase controls pathway choice in the resolution of lesion-stalled replisomes

This study shows how the replicative polymerase's proofreading subunit controls lesion resolution pathways in bacteria by restricting access to TLS polymerases.