Live-cell RNA imaging with the inactivated endonuclease Csy4 enables new insights into plant virus transport through plasmodesmata
D. Burnett, M. Hussein, Z. K. Barr, L. N. Nather, K. M. Wright, J. TILSNER
This paper introduces a live-cell RNA imaging system using an inactive Csy4 endonuclease to study plant virus transport through plasmodesmata, revealing RNA accessibility and non-specific recruitment of replication complexes.
bioRxiv · Jun 27, 2024Cell Biology