A. Marshall

Ectopic DNA integration and marker-free CRISPR/Cas9 strategies for the halotolerant black yeast Hortaea werneckii

This paper develops ectopic DNA integration and marker-free CRISPR/Cas9 methods for the halotolerant yeast Hortaea werneckii to enable genetic studies of its unique physiology and cell biology.

PI3K-dependant reprogramming of hexokinase isoforms controls glucose metabolism and functional responses of B lymphocytes

This study shows that PI3K-dependent upregulation of hexokinase 2 (HK2) controls glucose metabolism and immune functions in B cells, with HK2-deficient B cells showing impaired metabolic and functional responses.

Strategies for Inducing and Validating Zinc Deficiency and Zinc Repletion

This paper provides validated dietary strategies to modulate zinc homeostasis in mice and outlines methods to assess zinc status, enhancing reproducibility in disease-related studies.

3D Mitochondrial Structure in Aging Human Skeletal Muscle: Insights into MFN-2 Mediated Changes

This study investigates age-related changes in mitochondrial 3D structure in human skeletal muscle and shows that exercise can restore mitochondrial integrity through mitofusin 2.

Towards establishing a 24-hour, microplate-based, transcriptomics assay for rainbow trout embryos

This paper establishes a 24-hour, microplate-based transcriptomics assay for rainbow trout embryos to generate toxicological data efficiently and ethically.

Defining Mitochondrial Cristae Morphology Changes Induced by Aging in Brown Adipose Tissue

This study uses 3D electron microscopy to analyze mitochondrial cristae morphology in mouse brown adipose tissue across aging, finding significant structural changes in aged tissue.

Application of nanopore sequencing for accurate identification of bacterial colonies

This study compares Sanger and nanopore sequencing for identifying bacterial colonies from bioaerosol samples, finding nanopore sequencing more accurate in multispecies colonies.

ATF4 Dependent Increase in Mitochondrial-Endoplasmic Reticulum Tethering Following OPA1 Deletion in Skeletal Muscle

This study shows that OPA1 deficiency in skeletal muscle increases MERC formation via ATF4, affecting calcium exchange and mitochondrial-ER tethering.

DRP1-mediated mitochondrial fission is essential to maintain cristae morphology and bioenergetics

This study investigates how DRP1 mutations affect mitochondrial and peroxisomal dynamics, leading to metabolic dysfunction and structural abnormalities in EMPF1 patient fibroblasts.

The Role of Mentoring in Promoting Diversity, Equity, and Inclusion in STEM Education and Research

The paper discusses the importance of mentoring in STEM for underrepresented minorities and presents workshop feedback showing student satisfaction despite low initial expectations.

An effective workshop on How to be an Effective Mentor for Underrepresented STEM Trainees

This workshop provides a framework for effective mentorship of underrepresented STEM trainees to address systemic issues in academic pipelines.

Using a Champion-Oriented Mindset to Overcome the Challenges of Graduate School

The paper describes a workshop aimed at preparing minority students for graduate school by fostering a champion-oriented mindset, showing it was well-received and perceived as helpful.

Combining metabolomics and experimental evolution reveals key mechanisms underlying longevity differences in laboratory evolved Drosophila melanogaster populations

The paper combines metabolomics and experimental evolution to identify genetic and physiological mechanisms underlying longevity differences in Drosophila melanogaster populations.

Systematic Transmission Electron Microscopy-Based Identification and 3D Reconstruction of Cellular Degradation Machinery

This paper describes methods to identify and distinguish subcellular structures involved in macroautophagy using TEM and 3D reconstruction, with applications to quantify degradation machinery under different conditions.

Practices for Measuring 3D Organelle Morphology and Generating Surfaces with Amira

The paper introduces a standardized protocol using Amira software to quantify 3D organelle morphology from SBF-SEM data, demonstrating its application to mitochondria and ER structures.

A comprehensive approach for artifact-free sample preparation and assessment of mitochondrial morphology in tissue and cultured cells

This paper presents a method for preserving and quantitatively measuring mitochondrial morphology in tissues and cultured cells without introducing artifacts.

High-sensitivity monitoring of ctDNA by patient-specific sequencing panels and integration of variant reads

This paper introduces INVAR, a method to improve ctDNA detection sensitivity using patient-specific sequencing panels and variant read integration, achieving ppm-level detection in clinical samples.

RB1 deletion in RB-pathway disrupted cells results in DNA damage and cancer progression.

This study shows that RB1 mutations in already compromised RB-pathway cells increase DNA damage and cancer progression through genomic instability and impaired repair mechanisms.