B. Glancy

Overexpression of enhanced yellow fluorescent protein fused with Channelrhodopsin-2 causes contractile dysfunction in skeletal muscle

This study shows that overexpressing ChR2-EYFP in skeletal muscle causes contractile dysfunction, while ChR2-only mice show normal function, highlighting the need for alternative optogenetic models.

Impact of Capillary and Sarcolemmal Proximity on Mitochondrial Structure and Energetic Function in Skeletal Muscle

The paper compares mitochondrial structure and function in skeletal muscle based on proximity to capillaries or sarcolemma, finding capillary proximity enhances energy conversion and distribution.

Human Heart Failure Alters Mitochondria and Fiber 3D Structure Triggering Metabolic Shifts

This study identifies structural and metabolic changes in human heart failure using SBF-SEM and metabolomic analyses.

3D Mitochondrial Structure in Aging Human Skeletal Muscle: Insights into MFN-2 Mediated Changes

This study investigates age-related changes in mitochondrial 3D structure in human skeletal muscle and shows that exercise can restore mitochondrial integrity through mitofusin 2.

ATF4 Dependent Increase in Mitochondrial-Endoplasmic Reticulum Tethering Following OPA1 Deletion in Skeletal Muscle

This study shows that OPA1 deficiency in skeletal muscle increases MERC formation via ATF4, affecting calcium exchange and mitochondrial-ER tethering.

Loss of TMEM65 causes mitochondrial disease mediated by mitochondrial calcium

This study identifies TMEM65's role in mitochondrial calcium regulation, showing its loss causes mitochondrial disease and suggesting therapeutic targeting of mitochondrial calcium as a potential treatment.

3D Reconstruction of Murine Mitochondria Exhibits Changes in Structure Across Aging Linked to the MICOS Complex

This study examines age-related changes in mitochondrial 3D structure in mouse muscle and heart tissues, linking these changes to the MICOS complex and metabolic alterations.

Mitochondrial Network Configuration Influences Sarcomere and Myosin Filament Structure in Striated Muscles

The paper shows mitochondrial network configuration affects sarcomere and myosin filament structure in striated muscles across species.

Regulation of the Evolutionarily Conserved Muscle Myofibrillar Matrix by Cell Type Dependent and Independent Mechanisms

The paper shows that a myofibrillar matrix is conserved across species and regulated by both cell-type dependent and independent mechanisms in Drosophila muscles.

An Evolutionarily Conserved Regulatory Pathway of Muscle Mitochondrial Network Organization

The paper identifies an evolutionarily conserved pathway involving cut, salm, and H15 that regulates muscle mitochondrial network organization independently of contractile fiber-type in Drosophila.

Systematic Transmission Electron Microscopy-Based Identification and 3D Reconstruction of Cellular Degradation Machinery

This paper describes methods to identify and distinguish subcellular structures involved in macroautophagy using TEM and 3D reconstruction, with applications to quantify degradation machinery under different conditions.

Practices for Measuring 3D Organelle Morphology and Generating Surfaces with Amira

The paper introduces a standardized protocol using Amira software to quantify 3D organelle morphology from SBF-SEM data, demonstrating its application to mitochondria and ER structures.

A comprehensive approach for artifact-free sample preparation and assessment of mitochondrial morphology in tissue and cultured cells

This paper presents a method for preserving and quantitatively measuring mitochondrial morphology in tissues and cultured cells without introducing artifacts.

Reorganization of the Mitochondria-Organelle Interactome during Postnatal Development in Skeletal Muscle

This study shows how mitochondrial-organelle interactions reorganize during skeletal muscle development, shifting from inter-organelle communication to contractile specialization.

A Universal Approach to Analyzing Transmission Electron Microscopy with ImageJ

This paper introduces a standardized method using ImageJ to quantify subcellular organelle morphology in TEM images, focusing on mitochondria, ER, lysosomes, and autophagosomes.