DNA Thermo-Protection Facilitates Whole Genome Sequencing of Mycobacteria Direct from Clinical Samples by the Nanopore Platform
S. George, Y. Xu, G. Rodger, M. Morgan, N. D. Sanderson, S. Hoosdally, S. Thulborn, E. Robinson, P. Rathod, A. S. Walker, T. E. Peto, D. W. Crook, K. E. Dingle
This paper develops a thermo-protection buffer to enable direct whole genome sequencing of Mycobacteria from clinical samples using Nanopore technology, achieving high coverage at low bacterial concentrations.
bioRxiv · Apr 5, 2020 · PreprintMicrobiology