R. Allen

An arylsulphonamide that targets cell wall biosynthesis in Mycobacterium tuberculosis

The paper investigates an arylsulphonamide's mechanism against Mycobacterium tuberculosis, finding it targets cell wall biosynthesis via MmpL3.

Surface versus volume synthesis governs growth-dependent efficacy of a β-lactam antibiotic

This study shows that mecillinam affects the balance between surface area and volume synthesis in E. coli, leading to growth-rate dependent efficacy and lysis or sustained growth depending on the medium.

Engineering self-deliverable ribonucleoproteins for genome editing in the brain

This study engineers self-deliverable CRISPR ribonucleoproteins for efficient genome editing in neural progenitor cells and mouse brain tissue.

Siderophore synthetase-receptor gene coevolution reveals habitat- and pathogen-specific bacterial iron interaction networks

The paper develops a co-evolution algorithm to predict bacterial iron interaction networks based on siderophore synthetase-receptor gene pairs across Pseudomonas genomes, revealing habitat- and pathogen-specific interactions.

An in vitro experimental pipeline to characterize the binding specificity of SARS-CoV-2 neutralizing antibodies

This paper characterizes the binding specificity and epitope of a broadly neutralizing anti-SARS-CoV-2 Spike RBD antibody using mAb-resistant virions and cryo-EM structural analysis.

Genome editing in the mouse brain with minimally immunogenic Cas9 RNPs

This study demonstrates that minimally immunogenic Cas9 RNPs delivered via convection enhanced delivery can edit mouse striatal neurons with reduced immune responses compared to AAV-delivered Cas9.

Structural Optimization of siRNA Conjugates for Albumin Binding Achieves Effective MCL1-Targeted Cancer Therapy

This paper optimizes siRNA conjugates for albumin binding to enhance tumor delivery and efficacy in cancer therapy, achieving 80% MCL1 silencing and improved survival in breast cancer models.

A Systematic Survey of Reversibly Covalent Dipeptidyl Inhibitors of the SARS-CoV-2 Main Protease

This study systematically evaluates dipeptidyl inhibitors of SARS-CoV-2 main protease, identifying compounds with high antiviral potency and low cytotoxicity.

Chimeric mRNA based COVID-19 vaccine induces protective immunity against Omicron and Delta

This study develops a chimeric mRNA vaccine that provides broad protection against Omicron and Delta variants of SARS-CoV-2 by combining RBDs from different variants.

Discovery of a SARS-CoV-2 Broadly-Acting Neutralizing Antibody with Activity against Omicron and Omicron + R346K Variants

This paper identifies a broadly neutralizing antibody, STI-9167, effective against SARS-CoV-2 variants including Omicron and Omicron + R346K, and describes its development for clinical trials.

The N-Terminal Carbamate is Key to High Cellular and Antiviral Potency for Boceprevir-Based SARS-CoV-2 Main Protease Inhibitors

This paper identifies that a P4 N-terminal carbamate in boceprevir-based inhibitors is crucial for high antiviral potency against SARS-CoV-2.

The P3 O-Tert-Butyl-Threonine is Key to High Cellular and Antiviral Potency for Aldehyde-Based SARS-CoV-2 Main Protease Inhibitors

This study identifies O-tert-butyl-threonine at the P3 site as critical for enhancing cellular and antiviral potency of MPro inhibitors.

Cellular Activities of SARS-CoV-2 Main Protease Inhibitors Reveal Their Unique Characteristics

This paper evaluates the cellular potency of SARS-CoV-2 MPro inhibitors using a fluorescent flow cytometry assay, revealing that most inhibitors have weak cellular activity despite strong antiviral effects.

Tracking the stochastic growth of bacterial populations in microfluidic droplets

The paper presents a microfluidic device and imaging protocol to track stochastic bacterial growth in droplets, finding early growth statistics align with the Bellman-Harris model.

Biogenesis of RNA-containing extracellular vesicles at endoplasmic reticulum membrane contact sites

This study identifies VAP-A as a key regulator of RNA-containing extracellular vesicle biogenesis and shows its role in miR-100 transfer and tumor formation.

Jagged-1 regulates Foxp3 expression and cytokine production in CD4+ T cells

This study investigates how Jagged-1 influences Foxp3 expression and cytokine production in CD4+ T cells during interactions with dendritic cells.

Mechanism of Microbicidal Action of E-101 Solution, a Myeloperoxidase-Mediated Antimicrobial, and its Oxidative Products

This study evaluates the microbicidal action of E-101 solution and its oxidative products against MRSA and E. coli, showing rapid bactericidal activity and membrane damage.

Identifying mixed Mycobacterium tuberculosis infection and laboratory cross-contamination during Mycobacterial sequencing programs

The paper develops metrics to detect mixed Mycobacterium tuberculosis infections and cross-contamination in sequencing data from tuberculosis diagnostic labs.

Synchronous diversification of Sulawesi’s iconic artiodactyls driven by recent geological events

The paper tests the hypothesis that recent geological events on Sulawesi drove the synchronous diversification of its endemic artiodactyls, finding that species expanded from a central area that emerged above sea level ~1-2 Myr ago.