Author

S. Boutet

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

Click to filter author papers

Biochemistry· 3 Biophysics· 3 Cell Biology· 1 Plant Biology· 1

Signature Assays, Organisms & Methods

Click to filter · or open tag

Approach:experimental assay· 8 Research Focus:protein folding & dynamics· 4 Assay:structural biology· 3 Assay:metabolomics· 2 Assay:cryo-EM· 1 Research Focus:development & morphogenesis· 1 Research Focus:drug response / drug discovery· 1 Organism:E. coli· 1

Frequent Co-Authors

R. G. Sierra· 4 shared A. E. Cohen· 3 shared A. S. Brewster· 3 shared K. Tono· 3 shared M. C. Thompson· 3 shared N. K. Sauter· 3 shared S. Owada· 3 shared A. Aquila· 2 shared

Activity by Year

2019-2023 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 41 mutual co-author links

Click any collaborator node to inspect or filter shared papers

Focal author (center)Selected co-authorORCID co-authorUnlinked name

Curved chords = mutual co-authorship

4 shared papersBiochemistry

R. G. Sierra

Mutual co-authors in cluster

Author profile →

8 papers

Recent·Most cited

Changes in an Enzyme Ensemble During Catalysis Observed by High Resolution XFEL Crystallography

N. Smith, M. Dasgupta, D. C. Wych, C. Dolamore, R. G. Sierra, S. Lisova, D. Marchany-Rivera, A. E. Cohen, S. Boutet, M. S. Hunter, C. Kupitz, F. Poitevin, F. R. Moss, A. S. Brewster, N. K. Sauter, , M. A. Wilson

The paper uses XFEL crystallography to observe conformational changes in an enzyme during catalysis, revealing ionization-linked dynamics that control catalytic activity.

bioRxiv · Aug 15, 2023Biochemistry

Structural basis for functional properties of cytochrome c oxidase

I. Ishigami, R. G. Sierra, Z. Su, A. Peck, C. Wang, F. Poitevin, S. Lisova, B. Hayes, F. R. Moss, S. Boutet, R. E. Sublett, C. H. Yoon, S.-R. Yeh, D. L. Rousseau

This study reveals the structural differences between the oxidized O state and the metastable OH state of cytochrome c oxidase using spectroscopy and crystallography, showing how Y244's protonation state affects proton translocation.

bioRxiv · Mar 20, 2023 · PreprintBiophysics

ULTRAFAST STRUCTURAL CHANGES DIRECT THE FIRST MOLECULAR EVENTS OF VISION

T. Gruhl, T. Weinert, M. Rodrigues, C. J. Milne, G. Ortolani, K. Nass, E. Nango, S. Sen, P. J. Johnson, C. Cirelli, A. Furrer, S. Mous, P. Skopintsev, D. James, F. Dworkowski, , Scher

This study uses ultrafast crystallography to reveal how photoactivated retinal initiates structural changes in rhodopsin, leading to GPCR activation and vision.

bioRxiv · Oct 14, 2022 · PreprintBiochemistry

Metabolite profiling and cytotoxic activity of Andean potatoes: polyamines and glycoalkaloids as potential anticancer agents in human neuroblastoma cells in vitro

M. L. Lanteri, M. X. Silveyra, M. M. Moran, S. Boutet, D.-D. Solis-Gozar, F. Perreau, A. B. Andreu

This study investigates the composition and cytotoxic activity of Andean potato extracts against neuroblastoma cells, identifying polyamines and glycoalkaloids as potential anticancer agents.

bioRxiv · Oct 13, 2022Cell Biology

Rational control of structural off-state heterogeneity in a photoswitchable fluorescent protein provides switching contrast enhancement

V. Adam, K. Hadjidemetriou, N. Jensen, R. L. Shoeman, J. Woodhouse, A. Aquila, A.-S. Banneville, T. R. M. Barends, V. Bezchastnov, S. Boutet, M. Byrdin, M. Cammarata, S. Carbajo, N.-E. Christou, N. Coquelle, , Za

This paper enhances switching contrast in photoswitchable fluorescent proteins by modifying the V151 side chain, improving performance in RESOLFT nanoscopy.

ChemPhysChem · Aug 12, 2022Peer-reviewedBiophysics

Untargeted metabolomic analyses reveal the diversity and plasticity of the specialized metabolome in seeds of different Camelina sativa genotypes

S. Boutet, L. Barreda, F. Perreau, J.-C. Totozafy, C. Mauve, B. Gakiere, E. Delannoy, M.-L. Martin-Magniette, A. Monti, L. Lepiniec, F. Zanetti, M. Corso

This study analyzes the metabolomic diversity and plasticity of Camelina sativa seeds across genotypes and growing seasons, revealing environmental influences on specialized metabolites.

bioRxiv · Jan 18, 2021Plant Biology

Mix-and-inject XFEL crystallography reveals gated conformational dynamics during enzyme catalysis

M. DasGupta, D. Budday, S. H. P. de Oliveira, P. Madzelan, D. Marchany-Rivera, J. Seravalli, B. Hayes, R. G. Sierra, S. Boutet, M. Hunter, R. Alonso-Mori, A. Batyuk, J. Wierman, A. Lyubimov, A. S. Brewster, , M. A. Wilson

This study uses mix-and-inject XFEL crystallography to observe conformational changes in isocyanide hydratase during catalysis, showing how cysteine modification affects enzyme dynamics and function.

Proceedings of the National Academy of Sciences · Dec 4, 2019Peer-reviewedBiochemistry

Comparing serial X-ray crystallography and microcrystal electron diffraction (MicroED) as methods for routine structure determination from small macromolecular crystals

A. M. Wolff, I. D. Young, R. G. Sierra, A. S. Brewster, M. W. Martynowycz, E. Nango, M. Sugahara, T. Nakane, K. Ito, A. Aquila, A. Bhowmick, J. T. Biel, S. Carbajo, A. E. Cohen, S. Cortez, , M. C. Thompson

This study compares serial X-ray crystallography and MicroED for determining structures from small macromolecular crystals, highlighting their strengths and challenges.

bioRxiv · Sep 12, 2019Biophysics