S. Karki

The Sec61/TRAP Translocon Scrambles Lipids

The paper investigates the lipid scrambling activity of Sec61 and TRAP using molecular dynamics simulations, showing they facilitate lipid flip-flop via a credit card mechanism and are selective for phosphatidylcholine.

Collective heterogeneity of mitochondrial potential in contact inhibition of proliferation

This paper reveals a density-dependent collective metabolic heterogeneity in epithelial cells during contact inhibition of proliferation, showing distinct metabolic patterns in different stages.

Resolving ancient gene transfers clarifies the early co-evolution of eukaryotes and giant viruses

This study investigates gene exchange between giant viruses and eukaryotes, finding that some viral genes were acquired before the last eukaryotic common ancestor.

Molecular view of ER membrane remodeling by the Sec61/TRAP translocon

The paper determines the structure of the Sec61/TRAP complex bound to a ribosome using Cryo-EM, revealing how TRAP facilitates ER membrane remodeling and polypeptide translocation.

TGF-B signaling suppresses TCA cycle metabolism in renal cancer.

This study shows that TGF-β signaling suppresses TCA cycle enzyme expression in renal cancer, leading to reduced mitochondrial metabolism and tumor growth.

Comparative Genomics and Environmental Distribution of Large dsDNA viruses in the family Asfarviridae

This study analyzes Asfarviridae genomes and metagenomes to reveal their environmental distribution and genomic diversity across marine, freshwater, and terrestrial habitats.

Targeted knockdown of ribulose-1, 5-bisphosphate carboxylase-oxygenase in rice mesophyll cells impact on photosynthesis and growth

This study knocks down Rubisco small subunit genes in rice mesophyll cells, reducing Rubisco protein content and CO2 assimilation, leading to decreased biomass and grain yield.

A partial C4 photosynthetic biochemical pathway in rice

This paper introduces a partial C4 photosynthetic pathway in rice by expressing four C4 enzymes, showing increased 13C incorporation into malate and aspartate but limited flux into the Calvin-Benson cycle.

A role for neutral variation in the evolution of C4 photosynthesis

The paper shows natural variation in C4 enzyme expression in Arabidopsis and demonstrates that high C4 enzyme expression is neutral in rice, supporting the role of neutral variation in C4 photosynthesis evolution.

Risk factors associated with Avian Influenza subtype H9 outbreaks on poultry farms in Kathmandu valley, Nepal

This study identifies risk factors for AI subtype H9 outbreaks in poultry farms in Kathmandu Valley, Nepal, finding poor biosecurity and management practices are associated with increased risk.

Structural basis of SALM3 dimerization and synaptic adhesion complex formation with PTPσ

This study determines the structural basis of SALM3 dimerization and its interaction with PTPσ, showing that dimerization is required for synaptogenic activity.

The structure of SALM5 suggests a dimeric assembly for the presynaptic RPTP ligand recognition

The paper presents the crystal structure of SALM5's LRR-Ig domain and shows it forms a dimeric structure that binds RPTP ligands with micromolar affinity.