J. Ellenberg

A liquid-like coat mediates chromosome clustering during mitotic exit

This study shows Ki-67 switches from repelling to attracting chromosomes during mitotic exit, forming a liquid-like coat that drives chromosome clustering through phase separation.

Genetically encoded multimeric tags for intracellular protein localisation in cryo-EM

This paper introduces genetically encoded multimeric tags for localizing intracellular proteins in cryo-EM, demonstrating their use in human cells with drug-controlled labeling.

Pulsed stimulated Brillouin microscopy enables high-sensitivity mechanical imaging of live and fragile biological specimens

This paper introduces pulsed stimulated Brillouin microscopy to improve mechanical imaging of live biological specimens with reduced phototoxicity and enhanced signal-to-background ratio.

Assessment of 3D MINFLUX data for quantitative structural biology in cells revisited

The paper re-analyzes MINFLUX 3D imaging data to confirm its high localization precision and discuss its implications for structural biology.

High-resolution line-scan Brillouin microscopy for live-imaging of mechanical properties during embryo development

This paper introduces a high-resolution line-scan Brillouin microscope for live-imaging mechanical properties during embryo development with reduced photo-toxicity.

Rapid generation of homozygous fluorescent knock-in human cells using CRISPR/Cas9 genome editing and validation by automated imaging and digital PCR screening

This paper updates a CRISPR/Cas9 protocol for generating homozygous fluorescent knock-in human cells, improving efficiency and throughput through automation and digital PCR screening.

A quantitative map of nuclear pore assembly reveals two distinct mechanisms

The paper maps nuclear pore complex assembly in human cells, revealing two distinct molecular mechanisms with different orders of component addition.

Nanoscale 3D DNA tracing in single human cells visualizes loop extrusion directly in situ

This study uses nanoscale 3D imaging to show that DNA folds as flexible coils without loop extruding enzymes, but forms reproducible loops with Cohesin and CTCF.

3D super-resolution fluorescence microscopy maps the variable molecular architecture of the Nuclear Pore Complex

The paper uses 3D super-resolution microscopy and computational methods to map the 3D structure of the Nuclear Pore Complex in human cells, revealing variable conformations of its subunits.

Non-rodent mammalian zygotes assemble dual spindles despite the presence of paternal centrosomes

This study shows that non-rodent mammalian zygotes, like bovine and human, form two independent spindles around parental genomes despite inherited paternal centrosomes.

MINFLUX nanoscopy delivers multicolor nanometer 3D-resolution in (living) cells

The paper demonstrates MINFLUX nanoscopy achieves 1-3 nanometer 3D-resolution in living cells using a doughnut-shaped excitation beam and iterative localization.

Photoactivation of silicon rhodamines via a light-induced protonation

This paper introduces a new photoactivatable fluorophore that enables live-cell super-resolution microscopy without caging groups or side-products.

Direct visualization of single nuclear pore complex proteins using genetically-encoded probes for DNA-PAINT

This paper develops a method to directly visualize single nuclear pore complex proteins using DNA-PAINT microscopy with genetically-encoded tags, achieving ~3 nm resolution in human cells.

Nuclear pores as versatile reference standards for quantitative superresolution microscopy

The paper uses genome-edited cell lines with labeled nuclear pore complexes to serve as reference standards for quantitative superresolution microscopy.

Absolute quantification of cohesin, CTCF and their regulators in human cells

The paper measures absolute copy numbers and dynamics of cohesin, CTCF, and related proteins in human cells, revealing that some genomic enrichment sites are unoccupied in single cells.

Determining cellular CTCF and cohesin abundances to constrain 3D genome models

The paper quantifies CTCF and cohesin levels in mammalian cells to improve 3D genome models and provides a method for measuring Halo-tagged proteins.

Dual spindle formation in zygotes keeps parental genomes apart in early mammalian embryos

The paper shows that two spindles form in mammalian zygotes to keep parental genomes apart during the first cleavage.

A quantitative map of human Condensins provides new insights into mitotic chromosome architecture

The paper quantifies Condensin I and II dynamics during mitosis, proposing a three-step loop model for chromosome compaction.

Correlative live and super-resolution imaging reveals the dynamic structure of replication domains

The paper uses correlative live and super-resolution microscopy to study replication domain structure and dynamics in higher eukaryotes, finding RDs are 150 nm in size with specific spacing and mechanical coupling.

Postmitotic Nuclear Pore Assembly Proceeds By Radial Dilation Of Small ER Membrane Openings

The paper shows that postmitotic nuclear pore assembly occurs through dilation of ER membrane openings, forming transport channels rapidly.

Fast, robust and precise 3D localization for arbitrary point spread functions

The paper introduces a fast and precise 3D localization method for arbitrary PSFs, achieving high resolution and speed on standard microscopes.

Real-time chromatin dynamics at the single gene levelduring transcription activation

This study uses real-time imaging to show that transcription initiation rapidly confines a gene's chromatin domain, independent of RNA polymerase elongation.