J. Ries

Fast and robust 3D MINFLUX excitation with a variable phase plate

This paper introduces a fast and robust 3D MINFLUX excitation module using a variable phase plate, achieving record speed and performance.

Universal inverse modelling of point spread functions for SMLM localization and microscope characterization

This paper introduces uiPSF, a toolbox to infer accurate PSF models from microscopy data for improved SMLM imaging and microscope characterization.

Automated 3D multi-color single-molecule localization microscopy

This paper provides a protocol for setting up high-end 3D multi-color single-molecule localization microscopy (SMLM) in a laboratory setting.

Skp1 proteins are structural components of the synaptonemal complex in C. elegans

This study identifies SKR-1 and SKR-2 as structural components of the synaptonemal complex in C. elegans, independent of their role in the SCF ubiquitin ligase complex.

Field dependent deep learning enables high-throughput whole-cell 3D super-resolution imaging

This paper introduces FD-DeepLoc, a deep learning method for high-throughput 3D super-resolution imaging over a large field of view using a sCMOS camera.

Direct observation of motor protein stepping in living cells using MINFLUX

This study uses MINFLUX to track motor protein stepping in living cells and resolve their spatial organization on microtubules.

MicroFPGA: an affordable FPGA platform for microscope control

MicroFPGA provides an affordable FPGA-based platform for microscope control, enabling high-speed and parallel processing of complex control tasks.

Assessment of 3D MINFLUX data for quantitative structural biology in cells revisited

The paper re-analyzes MINFLUX 3D imaging data to confirm its high localization precision and discuss its implications for structural biology.

Nanoscale structural organization and stoichiometry of the budding yeast kinetochore

This study uses single-molecule localization microscopy to analyze the structure and stoichiometry of the budding yeast kinetochore, proposing a quantitative model based on nanoscale measurements.

Superresolution microscopy reveals partial preassembly and subsequent bending of the clathrin coat during endocytosis

The paper uses superresolution microscopy to show clathrin coats partially assemble on flat membranes before bending, and introduces a model to explain this process.

Maximum-likelihood model fitting for quantitative analysis of SMLM data

LocMoFit is an open-source framework that fits models to SMLM data using maximum likelihood estimation to extract biological insights from single-molecule localization coordinates.

Systematic Tuning of Rhodamine Spirocyclization for Super-Resolution Microscopy

This paper describes a method to systematically tune the spirocyclization equilibrium of rhodamines to create probes for different super-resolution microscopy techniques.

Photon-free (s)CMOS camera characterization for artifact reduction in high- and super-resolution microscopy

This paper develops an automated pipeline for sCMOS camera characterization using thermal signals, enabling artifact reduction in high- and super-resolution microscopy without additional hardware or user intervention.

3D super-resolution fluorescence microscopy maps the variable molecular architecture of the Nuclear Pore Complex

The paper uses 3D super-resolution microscopy and computational methods to map the 3D structure of the Nuclear Pore Complex in human cells, revealing variable conformations of its subunits.

SANS (USH1G) regulates pre-mRNA splicing by mediating the intra-nuclear transfer of tri-snRNP complexes

This study identifies SANS as a regulator of pre-mRNA splicing by facilitating the transfer of tri-snRNP complexes between Cajal bodies and nuclear speckles, linking it to Usher syndrome pathophysiology.

Deep learning enables fast and dense single-molecule localization with high accuracy

This paper introduces DECODE, a deep learning tool that improves single-molecule localization speed and density in SMLM with high accuracy across various imaging conditions.

Functionalized bead assay to measure 3-dimensional traction forces during T-cell activation

This paper develops a traction force microscopy platform to quantify local 3D traction forces exerted by T-cell microvilli during activation, linking force measurements to T-cell signaling.

SMAP - A Modular Superresolution Microscopy Analysis Platform for SMLM Data

SMAP provides a modular, open-source platform for analyzing SMLM data, enabling users to easily add custom algorithms and perform advanced analyses.

Direct Supercritical Angle Localization Microscopy for Nanometer 3D Superresolution

This paper introduces SALM, a 3D superresolution technique that improves z-resolution by four times using direct splitting of supercritical and undercritical emission.

Accurate 4Pi single-molecule localization using an experimental PSF model

The paper develops a method to improve 4Pi single-molecule localization microscopy by fitting an experimental PSF model, achieving the theoretical resolution limit.

Nanoscale pattern extraction from relative positions of sparse 3D localisations

The paper introduces a method to extract high-resolution ordered features from localisation microscopy data by analyzing relative molecular positions, applied to infer ultrastructure of various biological structures.

A cost-efficient open source laser engine for microscopy

The paper presents an open-source, cost-efficient laser engine for microscopy using affordable diodes and fiber delivery to reduce speckles.

MINFLUX nanoscopy delivers multicolor nanometer 3D-resolution in (living) cells

The paper demonstrates MINFLUX nanoscopy achieves 1-3 nanometer 3D-resolution in living cells using a doughnut-shaped excitation beam and iterative localization.

Photoactivation of silicon rhodamines via a light-induced protonation

This paper introduces a new photoactivatable fluorophore that enables live-cell super-resolution microscopy without caging groups or side-products.

Nanoscale subcellular architecture revealed by multicolor 3D salvaged fluorescence imaging

This paper introduces a super-resolution microscope that images whole mammalian cells at ~20 nm 3D resolution, revealing complex cellular structures previously only visible via electron microscopy.

Direct visualization of single nuclear pore complex proteins using genetically-encoded probes for DNA-PAINT

This paper develops a method to directly visualize single nuclear pore complex proteins using DNA-PAINT microscopy with genetically-encoded tags, achieving ~3 nm resolution in human cells.

Nuclear pores as versatile reference standards for quantitative superresolution microscopy

The paper uses genome-edited cell lines with labeled nuclear pore complexes to serve as reference standards for quantitative superresolution microscopy.

Depth-dependent PSF calibration and aberration correction for 3D single-molecule localization

This paper develops a method to correct depth-induced aberrations in 3D single-molecule localization microscopy by using an imperfect PSF model and post-processing error correction.

Type-I myosins promote actin polymerization to drive membrane bending in endocytosis

This study shows type-I myosins promote actin polymerization to drive membrane bending during endocytosis in budding yeast.

Super-resolution fight club: A broad assessment of 2D & 3D single-molecule localization microscopy software

The paper evaluates 36 single-molecule localization microscopy software packages using simulated datasets to assess their performance in 2D and 3D imaging.