S. Jakobs

Three-dimensional multi-target super-resolution microscopy of cells using Metal-Induced Energy Transfer and DNA-PAINT

This paper introduces MIET-PAINT, a method combining DNA-PAINT and Metal-Induced Energy Transfer to achieve nanometer-scale 3D imaging with high multiplexing capability.

In-situ architecture of the human prohibitin complex

This study determines the molecular organisation of the human prohibitin complex within the mitochondrial inner membrane using structural biology methods.

Endogenous BAX and BAK form mosaic rings of variable size and composition on apoptotic mitochondria

The paper shows that BAX and BAK form mosaic rings on apoptotic mitochondria, with BAK recruiting first and both proteins forming rings independently in knockout cells.

The Drosophila MIC10 orthologue has a propensity to polymerize into cristae-shaping filaments

This study identifies DmMIC10b as the major MIC10 orthologue in Drosophila, showing it polymerizes into cristae-shaping filaments that affect mitochondrial structure and fly lifespan.

Deep learning enables fast, gentle STED microscopy

This paper uses deep learning to denoise STED images, reducing photobleaching and photodamage by significantly lowering pixel dwell time.

Age-dependent structural reorganization of utricular ribbon synapses

This study examines age-related structural changes in utricular ribbon synapses in mice, finding age-dependent formation of floating ribbon clusters in type I vestibular hair cells.

Multi-color live-cell STED nanoscopy of mitochondria with a gentle inner membrane stain

This paper introduces a new fluorescent marker, PKMO, enabling 3D live-cell STED nanoscopy of mitochondria with improved photostability and reduced phototoxicity, allowing multiplexed imaging of mitochondrial dynamics and interactions.

Structural insights into crista junction formation by the Mic60-Mic19 complex

This study reveals the structural role of the Mic60-Mic19 complex in forming mitochondrial crista junctions by acting as a molecular strut that controls their architecture.

Optimal Precision and Accuracy in 4Pi-STORM using Dynamic Spline PSF Models

This paper develops a 4Pi-STORM microscope with dynamic spline PSF models to achieve high 3D resolution and accuracy in single molecule localization microscopy.

MINSTED fluorescence localization and nanoscopy

The paper introduces MINSTED, a STED-based super-resolution microscopy method that achieves high localization precision with reduced fluorophore bleaching, and demonstrates its application in imaging Mic60 proteins in human cells.

Correlative fluorescence microscopy, transmission electron microscopy and secondary ion mass spectrometry (CLEM-SIMS) for cellular imaging

This paper introduces a protocol combining correlative fluorescence microscopy, transmission electron microscopy, and secondary ion mass spectrometry (CLEM-SIMS) to analyze cellular structure, protein localization, and isotopic composition.

Local translation in synaptic mitochondria influences synaptic transmission

The paper uses an imaging approach to study mitochondrial translation in neurons, finding it occurs in dendrites and axons, correlates with synaptic activity, and affects synaptic function.

Live-cell STED microscopy of mitochondrial cristae

The paper uses STED microscopy to visualize mitochondrial cristae dynamics in living human cells using a Cox8A-SNAP-tag fusion protein.