Author

T. A. Brown

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Cell Biology· 5 Biochemistry· 3

Signature Assays, Organisms & Methods

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Approach:experimental assay· 8 Assay:fluorescence / live-cell microscopy· 5 Research Focus:cell signaling & trafficking· 3 Research Focus:cell type annotation· 3 Organism:human· 2 Assay:imaging· 2 Research Focus:differential expression· 1 Research Focus:drug response / drug discovery· 1

Frequent Co-Authors

L. D. Lavis· 5 shared A. N. Tkachuk· 4 shared J. B. Grimm· 4 shared R. Patel· 3 shared H. Choi· 2 shared J. Lippincott-Schwartz· 2 shared J. S. Marvin· 2 shared L. Xie· 2 shared

Activity by Year

2017-2024 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 25 mutual co-author links

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5 shared papersCell Biology

L. D. Lavis

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Author profile →

8 papers

Recent·Most cited

A series of spontaneously blinking dyes for super-resolution microscopy

K. L. Holland, S. E. Plutkis, T. A. Daugird, A. Sau, J. B. Grimm, B. P. English, Q. Zheng, S. Dave, F. Rahman, L. Xie, P. Dong, A. N. Tkachuk, T. A. Brown, R. H. Singer, Z. J. Liu, , L. D. Lavis

This paper introduces a series of spontaneously blinking dyes with adjustable duty cycles for improved single-molecule localization microscopy in cells and dense structures.

bioRxiv · Feb 23, 2024Cell Biology

A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions

R. J. Farrell, K. G. Bredvik, M. B. Hoppa, S. T. Hennegan, T. A. Brown, T. A. Ryan

The paper develops a ratiometric ER calcium sensor to enable quantitative comparisons of ER Ca2+ levels across cell types and subcellular regions, showing lower resting Ca2+ in neurons compared to fibroblasts.

bioRxiv · Feb 15, 2024 · PreprintCell Biology

Transforming chemigenetic bimolecular fluorescence complementation systems into chemical dimerizers using chemistry

P. Kumar, A. Gutu, A. Waring, T. A. Brown, L. D. Lavis, A. G. Tebo

The paper develops novel fluorogens that activate FAST proteins and convert splitFAST systems into chemical dimerizers, reducing the need for protein engineering.

bioRxiv · Dec 30, 2023 · PreprintBiochemistry

iATPSnFR2: a high dynamic range fluorescent sensor for monitoring intracellular ATP

J. S. Marvin, A. C. Kokotos, M. Kumar, C. Pulido, A. N. Tkachuk, J. S. Yao, T. A. Brown, T. A. Ryan

The paper develops iATPSnFR2, a high dynamic range fluorescent ATP sensor for real-time subcellular ATP monitoring, showing improved performance over previous versions.

bioRxiv · Aug 24, 2023Biochemistry

An engineered biosensor enables dynamic aspartate measurements in living cells

K. Davidsen, J. S. Marvin, A. Aggarwal, T. A. Brown, L. B. Sullivan

This paper develops a GFP-based biosensor to dynamically measure aspartate levels in living cells, showing it correlates with mass spectrometry measurements and detects temporal changes in aspartate concentration.

bioRxiv · Jun 27, 2023Biochemistry

Deuteration improves small-molecule fluorophores

J. B. Grimm, L. Xie, J. C. Casler, R. Patel, A. N. Tkachuk, H. Choi, J. Lippincott-Schwartz, T. A. Brown, B. S. Glick, Z. Liu, L. D. Lavis

This study shows that deuteration of small-molecule fluorophores enhances their brightness, chromostability, and photostability in cellular imaging.

bioRxiv · Aug 17, 2020 · PreprintCell Biology

Optimization and functionalization of red-shifted rhodamine dyes

J. B. Grimm, A. N. Tkachuk, H. Choi, B. Mohar, N. Falco, R. Patel, J. Lippincott-Schwartz, T. A. Brown, L. D. Lavis

This study optimizes red-shifted rhodamine dyes to improve their performance and enables functionalization with different chemical groups.

bioRxiv · Dec 20, 2019 · PreprintCell Biology

A general method to fine-tune fluorophores for live-cell and in vivo imaging

J. B. Grimm, A. K. Muthusamy, Y. Liang, T. A. Brown, W. C. Lemon, R. Patel, R. Lu, J. J. Macklin, P. J. Keller, N. Ji, L. D. Lavis

The paper introduces a method to fine-tune fluorophores for improved brightness and photostability in live-cell and in vivo imaging.

bioRxiv · Apr 14, 2017Cell Biology