L. D. Lavis

The paper develops novel fluorogens that activate FAST proteins and convert splitFAST systems into chemical dimerizers, reducing the need for protein engineering.

The paper uses correlative single molecule lattice light sheet imaging to study nucleosome dynamics and chromatin environment interactions in live cells, revealing differential diffusion and packing with increasing chromatin density.

This study investigates the dynamic search and processive nucleosome translocation mechanisms of RSC and ISW2 chromatin remodelers using optical tweezers and single-particle imaging.

The paper designs cell-permeable multifunctional ligands for HaloTag fusions that enable protein labeling and translocation, using Si-rhodamines with low KL-Z and high logD7.4 values.

This study uses live-cell imaging to show that PBAF chromatin remodeler dynamically targets acetylated chromatin hubs via bromodomains, with distinct binding kinetics in euchromatin and heterochromatin.

This study shows that deuteration of small-molecule fluorophores enhances their brightness, chromostability, and photostability in cellular imaging.

This study optimizes red-shifted rhodamine dyes to improve their performance and enables functionalization with different chemical groups.

This study introduces MoonTag, a fluorescence labeling system to visualize single mRNA translation, revealing stochastic start site selection and dynamic regulation in real-time.

The paper introduces a bleaching-independent STED microscopy method using exchangeable fluorophores, enabling long-term whole-cell 3D and multi-color imaging in live cells.

Spot-On provides a bias-corrected analysis tool for single-particle tracking experiments, outperforming existing methods and demonstrating robust inference of diffusion constants and subpopulations.

This paper introduces a genetic strategy for sparse protein labeling in live cells, enabling long-term single-molecule tracking and revealing new insights into molecular dynamics in vivo.

The paper shows that low-affinity enhancer binding by Ubx is efficient in nuclear microenvironments with high Ubx concentration, aided by cofactor Hth.

The paper introduces a method to fine-tune fluorophores for improved brightness and photostability in live-cell and in vivo imaging.

This paper develops semisynthetic pH-sensitive fluorophores that enable imaging of exocytosis and endocytosis, matching the performance of GFP-based pHluorin.