P. Dong

A series of spontaneously blinking dyes for super-resolution microscopy

This paper introduces a series of spontaneously blinking dyes with adjustable duty cycles for improved single-molecule localization microscopy in cells and dense structures.

Chromosome segment scanning for gain- or loss-of-function screening (CHASING) and its application in metabolic engineering

The paper introduces CHASING, a method for gain- or loss-of-function screening using chromosome-segment deletions, and applies it to identify metabolic engineering targets in B. subtilis.

Endothelial TMEM16F lipid scramblase regulates angiogenesis

This study identifies TMEM16F as a regulator of angiogenesis through intracellular signaling, showing impaired retinal angiogenesis in TMEM16F-deficient mice and altered VE-cadherin and Src kinase activity in human endothelial cells.

A pro-inflammatory stem cell niche drives myelofibrosis through a targetable galectin 1 axis

This study identifies a pro-inflammatory stem cell niche that drives myelofibrosis via galectin 1, offering a targetable pathway for therapy.

Nucleocapsid mutation R203K/G204R increases the infectivity, fitness and virulence of SARS-CoV-2

This study shows that the N protein mutations R203K/G204R increase SARS-CoV-2 infectivity, replication, and virulence, contributing to its spread and disease severity.

Quantifying E2F1 protein dynamics in single cells

This study quantifies E2F1 protein dynamics in single cells using fluorescent reporters, revealing cell cycle-dependent expression patterns and the impact of 3UTR sequences.

Phase separation of YAP reorganizes genome topology for long-term YAP target gene expression

This study shows YAP forms liquid-like condensates that reorganize genome topology to enable long-term target gene expression.

Visualizing Long-Term Single-Molecule Dynamics in vivo by Stochastic Protein Labeling

This paper introduces a genetic strategy for sparse protein labeling in live cells, enabling long-term single-molecule tracking and revealing new insights into molecular dynamics in vivo.