H. Choi

Diffusing protein binders to intrinsically disordered proteins

The paper develops RFdiffusion to generate protein binders for IDPs/IDRs, achieving nanomolar affinity and functional applications in amylin and IL2RG.

Spatial transcriptomics unveils landscape of resistance to concurrent chemo-radiotherapy in hypopharyngeal squamous cell carcinoma: the role of SPP1+ macrophages

This study uses spatial transcriptomics to identify SPP1+ macrophages as key contributors to CCRT resistance in hypopharyngeal SCC by modulating the tumor microenvironment.

PITPβ promotes COPI vesicle fission through lipid transfer and membrane contact formation

The paper identifies PITPβ's role in COPI vesicle fission by delivering phosphatidylcholine through membrane contacts, revealing a mechanism for lipid transfer in intracellular transport.

Dual-mode action of scalable, high-quality engineered stem cell-derived SIRPα-extracellular vesicles for treating acute liver failure

This paper develops SIRPα-expressing extracellular vesicles from engineered stem cells to treat acute liver failure by resolving necroptosis and promoting liver regeneration, showing reduced liver damage and improved survival in models.

Spatial Transcriptomics Reveals Spatially Diverse Cancer-Associated Fibroblast in Lung Squamous Cell Carcinoma Linked to Tumor Progression

This study uses spatial transcriptomics to analyze cancer-associated fibroblasts in lung squamous cell carcinoma, linking their spatial organization to tumor progression and patient outcomes.

Disruption of redox balance in glutaminolytic triple negative breast cancer by inhibition of glutamate export and glutaminase

This study shows that inhibiting glutaminase and xCT in TNBC disrupts redox balance, enhances chemotherapy efficacy, and can be monitored non-invasively using PET.

Lysosomal release of amino acids at ER three-way junctions regulates transmembrane and secretory protein mRNA translation

The paper shows that lysosomal amino acid release at ER junctions regulates translation of secretome mRNAs, with Lunapark and lysosomal function playing key roles.

Reproducing Left Ventricular Twist by Mimicking Myocardial Fiber Orientation Using 3D Bioprinting-assisted Tissue Assembly

This paper develops a 3D bioprinting-assisted method to create a chamber-like structure with myocardial fiber orientation, demonstrating left ventricular twist and structural-functional synchrony.

Unbiased phosphoproteomics analysis unveils modulation of insulin signaling by extramitotic CDK1 kinase activity in human myotubes

The paper uses phosphoproteomics to show that CDK1 activity modulates insulin signaling and glucose uptake in human myotubes, with group-specific phosphorylation changes observed.

LRRC8A anion channels modulate vasodilation via association with Myosin Phosphatase Rho Interacting Protein (MPRIP)

This study shows LRRC8A anion channels interact with MPRIP to regulate vascular relaxation and inflammation through redox-dependent cytoskeletal changes.

Mapping cell types in the tumor microenvironment from tissue images via deep learning trained by spatial transcriptomics of lung adenocarcinoma

The paper develops a deep learning method to map cell types in lung adenocarcinoma tumor microenvironments using H&E images trained on spatial transcriptomic data.

CD3e-immunotoxin spares CD62L(lo) Tregs and reshapes organ-specific T-cell composition by preferentially depleting CD3e(hi) T cells.

Complex changes in serum protein levels in COVID-19 convalescents

This study identifies changes in serum protein levels in COVID-19 convalescents compared to healthy controls, revealing both similarities and differences with acute phase changes.

Proteomic signatures of the serological response to flu vaccination in a large human cohort study

The paper identifies proteomic signatures associated with seroconversion after flu vaccination in a human cohort, revealing links between protein levels and immune response factors.

Targeted protein degradation reveals BET bromodomains as the cellular target of Hedgehog Pathway Inhibitor-1

This study identifies BET bromodomains as the target of HPI-1 using a PROTAC-based approach and shows HPP-9's mechanism of prolonged Hh pathway inhibition.

Time-resolved analysis of transcription kinetics in single live mammalian cells

The paper develops a method to measure transcription kinetics in live cells using time-lapse imaging and mathematical modeling, finding elongation rates and termination times for specific mRNAs.

Diverse northern Asian and Jomon-related genetic structure discovered among socially complex Three Kingdoms period Gaya region Koreans

The study reports the first ancient Korean genomes from the Three Kingdoms period, revealing genetic diversity linked to northern Chinese and Japanese Jomon-related ancestry.

Most primary olfactory neurons have individually neutral effects on behavior

The paper investigates the behavioral effects of individual olfactory receptor neurons in Drosophila and finds that most have neutral effects, but their combinations can produce strong behavioral responses.

Oxidant-Resistant LRRC8A/C Anion Channels Support Superoxide Production by Nox1

This study shows that LRRC8A/C anion channels regulate Nox1-mediated superoxide production and TNF signaling in vascular smooth muscle cells, with LRRC8C being more resistant to oxidants than LRRC8D.

Inhibiting DDX3X triggers tumor-intrinsic type I interferon response and enhances anti-tumor immunity

This study shows that inhibiting DDX3X in breast cancer cells increases type I interferon response and enhances anti-tumor immunity by promoting antigen presentation and T cell infiltration.

Deuteration improves small-molecule fluorophores

This study shows that deuteration of small-molecule fluorophores enhances their brightness, chromostability, and photostability in cellular imaging.

Primary platinum resistance and immune exclusion in ovarian carcinomas with high expression of the homologous recombination mediator RAD51

This study shows that high RAD51 expression in ovarian cancer is linked to poor survival and platinum resistance, particularly in HRD-negative tumors, and correlates with immune exclusion.

DNA micro-disk for the management of DNA-based data storage with index and write-once-read-many (WORM) memory features

This paper introduces DNA micro-disks with index and WORM memory features to improve DNA-based data storage reliability and accessibility.

Label-free single-instance protein detection in vitrified cells

This paper introduces a method to detect protein conformations in vitrified cells using high-resolution template matching, revealing insights into ribosome dynamics and ligand binding.

Identification of viruses from fungal transcriptomes

This study identifies mycoviruses from fungal transcriptomes, revealing their diversity, host distribution, and complex population dynamics.

Heterogeneity of proteome dynamics between connective tissue phases of adult tendon

The paper measures protein turnover rates in collagen and glycoprotein phases of rat Achilles tendon using isotope labeling and mass spectrometry, finding significant differences in proteome dynamics between the two ECM phases.

Optimization and functionalization of red-shifted rhodamine dyes

This study optimizes red-shifted rhodamine dyes to improve their performance and enables functionalization with different chemical groups.

Using force data to self-pace an instrumented treadmill and measure self-selected walking speed

This paper develops a self-pacing controller for force-instrumented treadmills to measure self-selected walking speed, showing it is accurate and faster than manual methods.

Vaccination with single plasmid DNA encoding IL-12 and antigens of severe fever with thrombocytopenia syndrome virus elicits complete protection in IFNAR knockout mice

This paper develops a DNA vaccine encoding SFTSV antigens and adjuvants that provides complete protection in IFNAR knockout mice, while protein antigens alone fail to do so.

Molecular determinants of chaperone interactions on MHC-I for folding and antigen repertoire selection

This paper identifies molecular determinants of TAPBPR chaperone interactions with MHC-I molecules, showing that conformational dynamics and disulfide bonds influence recognition and antigen repertoire selection.