T. Kiyono

Human intestinal organoid-derived PDGFRα+ mesenchymal stroma empowers LGR4+ epithelial stem cells

This study shows that organoid-derived mesenchymal stromal cells support the proliferation and functionality of hPSC-derived small intestinal epithelial stem cells in adherent culture.

Optineurin deficiency impairs autophagy to cause interferon beta overproduction and increased survival of mice following viral infection

This study shows that optineurin deficiency leads to IFNβ overproduction and increased survival in mice during viral infection due to impaired autophagy.

Drug metabolic activity as a selection factor for pluripotent stem cell-derived hepatic progenitor cells

This study isolates and expands hepatic progenitor cells from human ESCs by selecting for drug-metabolizing activity, enabling a stable supply of functional liver cells for therapy and drug testing.

Drug metabolism activity is a Critical Intrinsic Selector for Hepatocytes to elongate cell lifespan by using a cell-killing antibiotic

This study develops a method to expand human hepatocytes in vitro using a cell-killing antibiotic, enabling long-term culture and stable supply for drug research.

Puromycin selection of cells with a high expression of the cytochrome P450 CYP3A4 gene activity from a patient with drug-induced liver injury (DILI) and their lifespan prolongation using a combination of CDK4R24C, cyclin D1 and TERT

The paper generates an immortalized hepatocyte cell line with high CYP3A4 expression from a DILI patient and shows its prolonged lifespan and drug-metabolism capacity.

Ammonia-based enrichment and long-term propagation of zone I hepatocyte-like cells

This study shows that ammonia treatment enriches hepatocyte-like cells derived from human pluripotent stem cells, which can be propagated long-term in vitro while maintaining liver-specific gene expression.

Immortalization of human zone I hepatocytes from biliary atresia with CDK4R24C, cyclin D1, and TERT for cytochrome P450 induction testing

The paper describes the creation of an immortalized hepatocyte cell line, HepaMN, from biliary atresia tissue and demonstrates its utility for cytochrome P450 induction testing.