V. Hauryliuk

This study identifies the roles of ABCF ATPases YfmR and YkpA/YbiT in resolving ribosomal stalling during protein synthesis in Bacillus subtilis.

The paper shows that viral proteins activate PARIS-mediated tRNA degradation, and viral tRNAs rescue infection by preventing cleavage.

The paper identifies a core combinatorial network of Type II toxin-antitoxin systems, reveals their structural basis for hyperpromiscuity, and validates new TA systems with functional annotations.

This work uses deep learning to uncover novel protein families and folds by analyzing sequence and structural data, adding new entries to Pfam and identifying a novel toxin-antitoxin system.

The paper characterizes genome-encoded ABCF proteins in Gram-positive bacteria, showing their roles in intrinsic antibiotic resistance and revealing structural and regulatory mechanisms.

This study identifies a bacterial immune system that directly senses a viral capsid protein through a toxin-antitoxin system, revealing a PAMP-like mechanism for innate immunity in bacteria.

This study shows synthetic iboxamycin is effective against Listeria monocytogenes, overcoming resistance mechanisms, and highlights potential resistance development.

The paper investigates how deletions in SARS-CoV-2 Nsp1 affect protein stability and translation inhibition, finding that certain deletions reduce translation inhibition while others impact interferon response.

This paper presents cryo-EM structures of PoxtA bound to the ribosome, revealing its mechanism of antibiotic resistance by perturbing tRNA and altering nascent chain conformation.

The paper identifies and validates a hyperpromiscuous antitoxin domain, Panacea, capable of neutralizing diverse toxin domains across microbial and bacteriophage TA systems.

The paper investigates how four RSH toxins inhibit bacterial growth by pyrophosphorylating tRNA, disrupting protein synthesis and amino acid sensing.

The paper determines the structures of ARE ABCF-ribosome complexes from three Gram-positive bacteria and proposes a model for antibiotic resistance mechanisms.

The paper identifies Hfq-assisted RsmA regulation as critical for controlling PEL polysaccharide expression in Pseudomonas aeruginosa biofilms.

This study shows that the RRM domain in long RSH enzymes is essential for proper ribosome-mediated activation, preventing excessive (p)ppGpp production and cytotoxicity.

The paper reveals how RelA/SpoT Homolog factor Rel in Bacillus subtilis detects amino acid starvation by interacting with deacylated tRNA on the ribosome, triggering (p)ppGpp synthesis.

The paper identifies toxin-antitoxin systems in bacteria using alarmone signalling, showing how SASs function as toxins and their antitoxins counteract them.

The paper presents a method to measure intracellular nucleotide concentrations in bacteria using HPLC-HILIC with UV and MS detection, showing reliable quantification of >25 molecular species.

This study identifies New1 as a translation termination factor in yeast by showing it binds the ribosome like eEF3 and affects ribosome queuing at stop codons.

This study shows that disrupting genes encoding ribosome inactivating factors improves the activity of cell-free translational systems in Bacillus subtilis and Saccharomyces cerevisiae.

This study uses ribosome profiling to analyze the effects of eEF3 depletion in yeast, finding reduced ribosome density at stop codons and altered ribosome distribution without specific E-site stalling.